Mouse Strain information
APB ID 2
Added 25/11/2005
Last Edited 10/05/2014
Strain name: C57BL/6JSfdAnu-Card11unm/AnuApb
Nickname: Unmodulated
Strain designation name: C57BL/6JSfdAnu-Card11unm/AnuApb
Genetics
Strain Types Mutant strain
Coisogenic
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: caspase recruitment domain family, member 11  
Chromosome 5
Affected Gene Symbol Card11
Protein expression of altered gene: Normal
Genetic alteration of gene: ENU induced mutation. Misense, hypomorphic.
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1916978
Synonyms CARMA1, BIMP3
Allele Name unmodulated
Allele Symbol Card11unm
MGI Allele Accession ID MGI:3045963
Ensembl Gene ID: ENSMUSG00000036526
Transcript ID: ENSMUST00000085786
Transcript name: Card11-201
bp change: T to A at position 1235 on cDNA
Exon number: 7
Exon ID: ENSMUSE00000431473
cDNA position: 1235
Amino acid change: Leucine to Glutamine at position 305
Sequence:
1205CTGACTCAGACAAGGCCATCTTGGACATCCTGGAACATGACCGGAAGGAGGCGCTAGAGGAcDNA (reverse strand)
295P--D--S--D--K--A--I--L--D--I--L--E--H--D--R--K--E--A--L--E--Dprotein
Version: NCBI m37, Ensembl build 54
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Diminished antibody formation upon T cell-independent type 2 and T cell-dependent immunization. Diminished B cell NFκB activation and proliferation to antigen receptor engagement, but normal proliferation to TLR4 stimulation. Failure to form normal IgMlow IgDhi follicular B cells, and failure to form peritoneal B1 B cells. Diminished T cell NFκB activation and proliferation to TCR+CD28 stimulation. Spontaneous dermatitis accompanied by dermal mastocytosis and hyper-IgE syndrome.
Original Genetic Background C57BL/6JSfdAnu
Genetic Background Currently Maintained C57BL/6JSfdAnu
Coat Colour Black
Strain Images
Serum IgE concentration in un/un homozygotes (open circles) and wild-type controls.
Serum IgE concentration in un/un homozygotes (open circles) and wild-type controls.
Flow cytometric analysis of spleen cells
Flow cytometric analysis of spleen cells
Serum antibody titres to the indicated immunogens in un/un homozygotes (open circles) and wild-type controls (closed circles).
Serum antibody titres to the indicated immunogens in un/un homozygotes (open circles) and wild-type controls (closed circles).
Strain identification
How is this strain characterised? Genotyping
PCR protocols Carma-1 (Unmodulated)
Unmodulated (Pigsy)
Unmodulated (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • B cell
  • T cell
  • dermititis
  • ENU
  • Wellcome Trust
Additional Information Discovered by Wellcome Trust immunization and flow cytometry screen by Lauren Wilson, Edyta Kucharska & Carola Vinuesa (JCSMR, ANU). Mutation mapped and identified by Dr Jesse Jun. Project guided by Prof Chris Goodnow.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 14 mice)

APB stock genotyped verified by: The APF
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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