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Mouse Strain information

APB ID

Added

25/11/2005

Last Edited

10/05/2014

Strain nomenclature:

C57BL/6JSfdAnu-Card11unm/AnuApb

Nickname:

Unmodulated

Strain designation name:

C57BL/6JSfdAnu-Card11unm/AnuApb

Genetics

Strain Types

Mutant strain
Coisogenic

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: caspase recruitment domain family, member 11

Chromosome

Affected Gene Symbol

Card11

Protein expression of altered gene:

Normal

Genetic alteration of gene:

ENU induced mutation. Misense, hypomorphic.

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1916978

Synonyms

CARMA1, BIMP3

Allele Name

unmodulated

Allele Symbol

Card11unm

MGI Allele Accession ID

MGI:3045963

Ensembl Gene ID:

ENSMUSG00000036526

Transcript ID:

ENSMUST00000085786

Transcript name:

Card11-201

bp change:

T to A at position 1235 on cDNA

Exon number:

Exon ID:

ENSMUSE00000431473

cDNA position:

1235

Amino acid change:

Leucine to Glutamine at position 305

Sequence:
1205	CTGACTCAGACAAGGCCATCTTGGACATCCTGGAACATGACCGGAAGGAGGCGCTAGAGGA	cDNA (reverse strand)
295	P--D--S--D--K--A--I--L--D--I--L--E--H--D--R--K--E--A--L--E--D	protein

Version:

NCBI m37, Ensembl build 54

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Diminished antibody formation upon T cell-independent type 2 and T cell-dependent immunization. Diminished B cell NFκB activation and proliferation to antigen receptor engagement, but normal proliferation to TLR4 stimulation. Failure to form normal IgMlow IgDhi follicular B cells, and failure to form peritoneal B1 B cells. Diminished T cell NFκB activation and proliferation to TCR+CD28 stimulation. Spontaneous dermatitis accompanied by dermal mastocytosis and hyper-IgE syndrome.

Original Genetic Background

C57BL/6JSfdAnu

Genetic Background Currently Maintained

C57BL/6JSfdAnu

Coat Colour

Black

Strain Images

Serum IgE concentration in un/un homozygotes (open circles) and wild-type controls.

Flow cytometric analysis of spleen cells

Serum antibody titres to the indicated immunogens in un/un homozygotes (open circles) and wild-type controls (closed circles).

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

Unmodulated (amplifluor)
Unmodulated (Pigsy)
Carma-1 (Unmodulated)

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Yes	Yes
Are homozygous mice fertile?	Yes	Yes
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Homozygous Matings Required?	No
Male Fertility Compromised?	Yes
Average Number of Pups at Birth	5.0
Average Number of Pups Surviving to Weaning	5.0
Weaning Age	21 days
Breeding Performance	Poor

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

B cell
T cell
dermititis
ENU
Wellcome Trust

Additional Information

Discovered by Wellcome Trust immunization and flow cytometry screen by Lauren Wilson, Edyta Kucharska & Carola Vinuesa (JCSMR, ANU). Mutation mapped and identified by Dr Jesse Jun. Project guided by Prof Chris Goodnow.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 14 mice)

APB stock genotyped verified by:

The APF

Are live mice available?

Unknown

MTA needs to be signed?

Yes