Home - Australian Phenomics Facility

Mouse Strain information

APB ID

158

Added

07/03/2006

Last Edited

09/05/2014

Strain nomenclature:

C57BL/6SfdAnu-Zap70mrtle/AnuApb

Nickname:

Mr T-Less

Strain designation name:

C57BL/6SfdAnu-Zap70mrtle/AnuApb

Genetics

Strain Types

Mutant strain
Coisogenic

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: Zap70, zeta-chain (TCR) associated protein kinase

Chromosome

Affected Gene Symbol

Zap70

Protein expression of altered gene:

Reduced

Genetic alteration of gene:

ENU-induced mutation. T⇒C at nucleotide 1601; W504R

Location on chromosome (bp):

36818706-36839661 bp, + strand (From NCBI annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:99613

Synonyms

Zap-70, Srk, TZK

Allele Name

Mr T-Less

Allele Symbol

Zap70mrtle

MGI Allele Accession ID

MGI:3614790

Ensembl Gene ID:

ENSMUSG00000026117

Transcript ID:

ENSMUST00000027291

Transcript name:

Zap70-201

bp change:

T to C at position 1601 on cDNA

Exon number:

Exon ID:

ENSMUSE00000230099

cDNA position:

1601

Amino acid change:

Tryptophan to Arginine at position 504

Sequence:
1571	GCCCGGTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCGCCAGAGTGCATCAACTTTCGGA	cDNA (forward strand)
494	-A--R--S--A--G--K--W--P--L--K--W--Y--A--P--E--C--I--N--F--R--	protein

Version:

NCBI m37, Ensembl build 55

Mutant Construction Technique

None

Candidate Gene

(From Ensembl annotation of NCBI Build 36)

Phenotype

Phenotype Homozygous State

Identified by severe reduction in T cell numbers in blood. B cells are normal Both CD4⁺ and CD8⁺ T cell populations were almost completely absent in peripheral blood. Therefore, no αβ T cells but have increase in γδ T cells Normal numbers of DN and DP T cells. Implies that have block at DP stage and mice unable to perform positive thymocyte selection. Intrinsic to T cells not microenvirnment. Cells unable to pass thymic selection steps. Cells unable to up-regulate TCR Reduced signalling through TCR. Zap70 protein expression of mrt/mrt thymocytes was 25% of wildtype thymocytes. Kinase domain affected by mutation, kinase assays not performed Heterozygous mice, mrt/+ have normal levels of CD4⁺ and CD8⁺ thymocytes but Zap70 protein expression is 2 fold lower. Unlike cells devoid of ZAP-70, mrtless thymocytes showed partial induction of CD5 and CD69, and were sensitive to TCR stimulation with a dose-response shifted approximately 10-fold. However, essentially no T cells were able to compensate for the mrtless mutation and mature beyond the CD4⁺ CD8⁺ stage.

Original Genetic Background

C57BL/6

Genetic Background Currently Maintained

C57BL/6

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

MrTless (amplifluor)
Mr T-Less (MrT)

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Yes

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

T cell
zap70
B cell
TCR
lymphocyte
ENU

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 4 mice)

APB stock genotyped verified by:

The APF

Are live mice available?

MTA needs to be signed?

Yes