Mouse Strain information
APB ID 158
Added 07/03/2006
Last Edited 09/05/2014
Strain name: C57BL/6SfdAnu-Zap70mrtle/AnuApb
Nickname: Mr T-Less
Strain designation name: C57BL/6SfdAnu-Zap70mrtle/AnuApb
Genetics
Strain Types Mutant strain
Coisogenic
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: Zap70, zeta-chain (TCR) associated protein kinase  
Chromosome 1
Affected Gene Symbol Zap70
Protein expression of altered gene: Reduced
Genetic alteration of gene: ENU-induced mutation. T⇒C at nucleotide 1601; W504R
Location on chromosome (bp): 36818706-36839661 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:99613
Synonyms Zap-70, Srk, TZK
Allele Name Mr T-Less
Allele Symbol Zap70mrtle
MGI Allele Accession ID MGI:3614790
Ensembl Gene ID: ENSMUSG00000026117
Transcript ID: ENSMUST00000027291
Transcript name: Zap70-201
bp change: T to C at position 1601 on cDNA
Exon number: 11
Exon ID: ENSMUSE00000230099
cDNA position: 1601
Amino acid change: Tryptophan to Arginine at position 504
Sequence:
1571GCCCGGTCTGCAGGGAAGTGGCCTCTGAAGTGGTACGCGCCAGAGTGCATCAACTTTCGGAcDNA (forward strand)
494-A--R--S--A--G--K--W--P--L--K--W--Y--A--P--E--C--I--N--F--R--protein
Version: NCBI m37, Ensembl build 55
Mutant Construction Technique None
Candidate Gene (From Ensembl annotation of NCBI Build 36)
Phenotype
Phenotype Homozygous State Identified by severe reduction in T cell numbers in blood. B cells are normal Both CD4⁺ and CD8⁺ T cell populations were almost completely absent in peripheral blood. Therefore, no αβ T cells but have increase in γδ T cells Normal numbers of DN and DP T cells. Implies that have block at DP stage and mice unable to perform positive thymocyte selection. Intrinsic to T cells not microenvirnment. Cells unable to pass thymic selection steps. Cells unable to up-regulate TCR Reduced signalling through TCR. Zap70 protein expression of mrt/mrt thymocytes was 25% of wildtype thymocytes. Kinase domain affected by mutation, kinase assays not performed Heterozygous mice, mrt/+ have normal levels of CD4⁺ and CD8⁺ thymocytes but Zap70 protein expression is 2 fold lower. Unlike cells devoid of ZAP-70, mrtless thymocytes showed partial induction of CD5 and CD69, and were sensitive to TCR stimulation with a dose-response shifted approximately 10-fold. However, essentially no T cells were able to compensate for the mrtless mutation and mature beyond the CD4⁺ CD8⁺ stage.
Original Genetic Background C57BL/6
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
PCR protocols MrTless (amplifluor)
Mr T-Less (MrT)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? Yes
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • T cell
  • zap70
  • B cell
  • TCR
  • lymphocyte
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 4 mice)

APB stock genotyped verified by: The APF
Are live mice available? No
MTA needs to be signed? Yes


   

 


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