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Mouse Strain information

APB ID

157

Added

06/03/2006

Last Edited

29/08/2014

Strain nomenclature:

C57BL/6JSfdAnu-Ptprcloc/AnuApb

Nickname:

Lochy

Strain designation name:

C57BL/6JSfdAnu-Ptprcloc/AnuApb

Genetics

Strain Types

Mutant strain
Coisogenic

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: Protein tyrosine phosphatase, receptor type, C

Chromosome

Affected Gene Symbol

Ptprc

Protein expression of altered gene:

Nil

Genetic alteration of gene:

ENU-induced mutation. Intronic T⇒A, nucleotide 139980367 in intron 20; results in 5 bp insertion ATCAG after nucleotide 2109 of mRNA, results in frame shift in Ptprc coding sequence, introducing 26 new amino acids and premature termination

Location on chromosome (bp):

139959992-140071843 bp, - strand (From Ensembl annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97810

Synonyms

B220, CD45, CD45R, Ly-5

Allele Name

Lochy

Allele Symbol

Ptprcloc

MGI Allele Accession ID

MGI:3611894

Ensembl Gene ID:

ENSMUSG00000026395

Transcript ID:

ENSMUST00000027645

Transcript name:

Ptprc-201

bp change:

T to A

Intron number:

Genomic position:

139980367

Sequence:
139980397	tccataaatgactgttctctttgtgtgtttTgatcagGGCTTCAAGGAACCCAGGAAATACA	genomic (reverse strand)
	Exons

Version:

NCBI m37, Ensembl build 55

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Decreased expression of CD45 on CD19⁺ B cells loc/loc mice have normal numbers of B cells Cell intrinsic defect Slightly reduced levels of IgM⁺IgD⁻, IgM⁺IgD⁺ and IgD⁺CD21⁺ cells Reduced levels of B220loCD19⁺ cells but normal levels of B220⁺CD19⁺ in bone marrow 3 fold decrease in DP thymocytes 10 fold decrease in CD4 SP thymocytes 5 fold decrease in CD8 SP thymocytes DP thymocytes had 2.5 fold increase in expression of CD4 and CD8, cell intrinsic Co-receptor expression on SP T cells was increased 1.5 fold Dramatic decrease in CD8⁺CD44lo/med cells TCRβ is up-regulated on DP thymocytes and down regulated on mature T cells. CD5 is down-regulated on DP thymocytes and up-regulated on peripheral T cells. Decrease in DP thymocytes is not cell intrinsic Decrease in SP thymocytes is cell intrinsic The decrease of CD69 and CD5 on thymocytes in thymus was cell intrinsic No CD45 protein visually detected by Western blotting Inhibition of DN cells progressing to DP Block preventing DP thymocytes maturing to SP T cells attributed to defective thymic selection Decrease in peripheral T cells Reduction in naïve CD8+ T cells and shift to activated/memory CD8⁺ T cell phenotype Lack of TCRβhi and CD69⁺ cells in thymus-implies defect in TCR signalling for positive selection

Original Genetic Background

C57BL/6JSfdAnu

Genetic Background Currently Maintained

C57BL/6JSfdAnu

Coat Colour

Black

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

Lochy (amplifluor)

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

General information

Associated IP rights?

Yes

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

CD45
T cell
B cell
CD8
lymphocyte
ENU

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 11 mice)

APB stock genotyped verified by:

The APF

Are live mice available?

MTA needs to be signed?

Yes