Mouse Strain information
APB ID 157
Added 06/03/2006
Last Edited 29/08/2014
Strain name: C57BL/6JSfdAnu-Ptprcloc/AnuApb
Nickname: Lochy
Strain designation name: C57BL/6JSfdAnu-Ptprcloc/AnuApb
Genetics
Strain Types Mutant strain
Coisogenic
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: Protein tyrosine phosphatase, receptor type, C  
Chromosome 1
Affected Gene Symbol Ptprc
Protein expression of altered gene: Nil
Genetic alteration of gene: ENU-induced mutation. Intronic T⇒A, nucleotide 139980367 in intron 20; results in 5 bp insertion ATCAG after nucleotide 2109 of mRNA, results in frame shift in Ptprc coding sequence, introducing 26 new amino acids and premature termination
Location on chromosome (bp): 139959992-140071843 bp, - strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97810
Synonyms B220, CD45, CD45R, Ly-5
Allele Name Lochy
Allele Symbol Ptprcloc
MGI Allele Accession ID MGI:3611894
Ensembl Gene ID: ENSMUSG00000026395
Transcript ID: ENSMUST00000027645
Transcript name: Ptprc-201
bp change: T to A
Intron number: 20
Genomic position: 139980367
Sequence:
139980397tccataaatgactgttctctttgtgtgtttTgatcagGGCTTCAAGGAACCCAGGAAATACAgenomic (reverse strand)
    Exons
Version: NCBI m37, Ensembl build 55
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Decreased expression of CD45 on CD19⁺ B cells loc/loc mice have normal numbers of B cells Cell intrinsic defect Slightly reduced levels of IgM⁺IgD⁻, IgM⁺IgD⁺ and IgD⁺CD21⁺ cells Reduced levels of B220loCD19⁺ cells but normal levels of B220⁺CD19⁺ in bone marrow 3 fold decrease in DP thymocytes 10 fold decrease in CD4 SP thymocytes 5 fold decrease in CD8 SP thymocytes DP thymocytes had 2.5 fold increase in expression of CD4 and CD8, cell intrinsic Co-receptor expression on SP T cells was increased 1.5 fold Dramatic decrease in CD8⁺CD44lo/med cells TCRβ is up-regulated on DP thymocytes and down regulated on mature T cells. CD5 is down-regulated on DP thymocytes and up-regulated on peripheral T cells. Decrease in DP thymocytes is not cell intrinsic Decrease in SP thymocytes is cell intrinsic The decrease of CD69 and CD5 on thymocytes in thymus was cell intrinsic No CD45 protein visually detected by Western blotting Inhibition of DN cells progressing to DP Block preventing DP thymocytes maturing to SP T cells attributed to defective thymic selection Decrease in peripheral T cells Reduction in naïve CD8+ T cells and shift to activated/memory CD8⁺ T cell phenotype Lack of TCRβhi and CD69⁺ cells in thymus-implies defect in TCR signalling for positive selection
Original Genetic Background C57BL/6JSfdAnu
Genetic Background Currently Maintained C57BL/6JSfdAnu
Coat Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols Lochy (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
General information
Associated IP rights? Yes
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • CD45
  • T cell
  • B cell
  • CD8
  • lymphocyte
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 11 mice)

APB stock genotyped verified by: The APF
Are live mice available? No
MTA needs to be signed? Yes


   

 


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