An NLS-cre gene was inserted into the endogenous ATG start site of the gene. An frt-flanked neomycin cassette was removed in ES cells via Flp-mediated recombination prior to production of chimeric mice.
Location on chromosome (bp):
Chr10:116714597-116719328 bp, - strand
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
The L1L2_Bact_P cassette was inserted at position 7164074 of Chromosome 15 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 7164933. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created.
Location on chromosome (bp):
7129572-7197489 bp, + strand From NCBI annotation of GRCm38
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex
carrying an inserted transgene with no homologous allele on the other chromosome.
