Mouse Strain information
APB ID 34
Added 20/04/2004
Last Edited 10/05/2014
Strain nomenclature: C57BL/6JSfdAnu-Dock8cpm/AnuApb
Nickname: Captain Morgan, ENU4AT:038
Strain designation name: C57BL/6JSfdAnu-Dock8cpm/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: dedicator of cytokinesis 8  
Chromosome 19
Affected Gene Symbol Dock8
Protein expression of altered gene: Nil
Genetic alteration of gene: ENU-induced mutation. The mutation comprises a T to C transition that modifies the splice donor sequence of exon 20, resulting in aberrant mRNA splicing between cryptic splice donors and the normal exon 21 splice acceptor. cDNA sequence analysis has identified the abnormal splice products: frameshifted mRNAs containing premature translation stop codons that terminate the protein before the DOCK-homology region 2 (DHR2) domain. DHR2 is known to bind to G proteins of the Rho family, promoting GTP -- GDP exchange and initiating a variety of cellular responses.
Location on chromosome (bp): 25074019-25276922 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:1921396
Allele Name dedicator of cytokinesis 8; captain morgan
Allele Symbol Dock8cpm
MGI Allele Accession ID MGI:3835422
Ensembl Gene ID: ENSMUSG00000052085
Transcript ID: ENSMUST00000025831
Transcript name: Dock8-201
bp change: T to C
Intron number: 20
Genomic position: 25202202
Sequence:
25202172GCAGCCCATGGTCATTGCTGGCCAAACAGgTagaggcctgtgggtagggaggaggcatggctgenomic (forward strand)
    Exons
Version: NCBI m37, Ensembl build 57
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Defective antibody response to immunization: failure to sustain antibody formation, undergo affinity maturation or to mount heightened recall (memory) response upon booster immunization. DOCK8-mutant B cells were unable to form marginal zone B cells or to persist in germinal centers and undergo affinity maturation. Dock8 mutations disrupted accumulation of the integrin ligand ICAM-1 in the B cell immunological synapse but did not alter other aspects of B cell antigen receptor signaling.
Original Genetic Background C57BL/6JSfdAnu
Genetic Background Currently Maintained C57BL/6JSfdAnu
Strain Images
Captain morgan homozygous mutant mice (cpm/cpm), primurus homozygous mice (pri/pri), compound heterozygotes between the two strains (cpm/pri), and wild-type control mice (+/+) were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS). Antibodies to CGG and to ARS were measured in serum of individual mice (dots) by ELISA, 14 and 28 days after primary immunization. Antibody titres are shown relative to a reference immune serum pooled from wild-type mice.
Captain morgan homozygous mutant mice (cpm/cpm), primurus homozygous mice (pri/pri), compound heterozygotes between the two strains (cpm/pri), and wild-type control mice (+/+) were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS). Antibodies to CGG and to ARS were measured in serum of individual mice (dots) by ELISA, 14 and 28 days after primary immunization. Antibody titres are shown relative to a reference immune serum pooled from wild-type mice.
Strain identification
How is this strain characterised? Genotyping
PCR protocols Cpt Morgan (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • gammaglobulin
  • immunization
  • antibody
  • ENU
  • Wellcome Trust
  • memory
  • B cell
  • GTP
Additional Information Discovered by Wellcome Trust Strategic Award. Identified in immunization antibody response screen by Ms Edyta Kucharska and Dr Carola Vinuesa (JCSMR, ANU), mapped and mutation identified by Mr Andy Johnson and Dr Teresa Lambe (Oxford University), selective defect in antibody maturation established by Dr Katrina Randall (JCSMR, ANU). Project guided by Prof Richard Cornall and Prof Chris Goodnow.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 3 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


     

 


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