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Mouse Strain information

APB ID

2369

Added

14/12/2007

Last Edited

09/05/2014

Strain nomenclature:

C57BL/6JSfdAnu-Prkcbtilc/AnuApb

Nickname:

Tilcara ; ENU6WT:121

Strain designation name:

C57BL/6JSfdAnu-Prkcbtilc/AnuApb

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Semi-dominant

1. Affected Gene Name: protein kinase C, beta 1

Chromosome

Affected Gene Symbol

Prkcb1

Protein expression of altered gene:

Unknown

Genetic alteration of gene:

ENU-induced mutation. T ➞C in Exon 15 resulting in S 552➡P

Location on chromosome (bp):

129432265-129777916 bp, + strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97596

Synonyms

PKC-Beta, Pkcb, Prkcb2

Allele Name

tilcara

Allele Symbol

Prkcbtilc

MGI Allele Accession ID

MGI:4819152

Allele synonyms:

ENU6WT:121

Ensembl Gene ID:

ENSMUSG00000052889

Transcript ID:

ENSMUST00000064989

Transcript name:

Prkcb-001

bp change:

T to C at position 1874 on cDNA

Exon number:

Exon ID:

ENSMUSE00000439236

cDNA position:

1874

Amino acid change:

Serine to Proline at position 552

Sequence:
1844	GAAGGGGAGGATGAGGATGAACTCTTCCAGTCAATCATGGAACACAATGTGGCGTATCCCA	cDNA (forward strand)
542	-E--G--E--D--E--D--E--L--F--Q--S--I--M--E--H--N--V--A--Y--P--	protein

Version:

NCBI m37, Ensembl build 56

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Defective T-cell independent type 2 antibody response to NP-Ficoll.

Original Genetic Background

C57BL/6JSfdAnu

Genetic Background Currently Maintained

C57BL/6JSfdAnu

Coat Colour

Black

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

Tilcara (Amplifluor)
Tilcara_(2007) New Hot Start

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

B cell
protein kinase
signal transduction
ENU
Wellcome Trust

Additional Information

Discovered by Wellcome Trust Immunization Screen. Identified in immunization antibody response screen by Ms Edyta Kucharska and Dr Anselm Enders (JCSMR, ANU). Mutation mapped and identified by Charis Teh, Belinda Whittle and Dr Anselm Enders. Project guided by Prof Chris Goodnow.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 6 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?

Yes