Sperm Cryopreservation
Funding from both CRIS allows us to offer mouse cryopreservation service for less than the actual cost for strains that are in the public domain, i.e. published, or available to other researchers. The ongoing maintenance costs, e.g. liquid nitrogen, are also supported.
To encourage cryopreservation of unpublished strains a 2 year embargo is permitted on listing on the APB database and distribution to others. At the completion of the 2 years the embargo must be lifted or the depositor must pay for the full cryopreservation costs plus maintenance fee as outlined in the second table below.
The depositor will need to pay for shipping of the mice to the APB node and the caging of the mice until cryopreservation.
The depositor will be required to cover costs associated with breeding mice to produce donor animals for embryo cryopreservation.
All costs are in AUD, are exclusive of GST and are subject to change at any time.
Unrestricted Strains
For strains that are in the public domain, i.e. published, or available to other researchers.
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Cost per strain |
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- Consumables individually identified with a unique barcode
- Cryopreservation of 5 mice
- Sample Storage at duplicate locations
- Data Storage
- Quality Control (QC) post thaw microscope analysis of the following parameters:
- Concentration
- Motility
- Progressive Motility
- Morphology (Gross defects)
- Cryopreservation report
- Analysis report
- Short term caging at cryopreservation facility when required
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- Shipping of animals to the
cryopreservation facility |
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- Consumables
- IVF
- Report with the following results:
- 2-cell fertilisation rate
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- Genotyping |
Restricted Strains
For those strains that are not freely distributable depositors will be charged for the cryopreservation, on going maintenance (a yearly fee) and for shipment.
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Cost per strain |
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$850 +GST |
- Consumables individually identified with a unique barcode
- Cryopreservation
- Sample Storage
- Data storage
- Quality Control (QC) post thaw microscope analysis of the following parameters:
- Concentration
- Motility
- Progressive Motility
- Morphology (Gross defects)
- Cryopreservation report
- Analysis report
|
- Shipping of animals to the
cryopreservation facility |
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$125 +GST |
- Duplicate storage at 2 locations |
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$850 +GST |
- Consumables
- IVF
- Report with the following results:
- 2-cell fertilisation rate
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- Genotyping |
Reanimation from Archived Sperm
Please see the Reanimation Page for more information on the reanimation services offered by the APB. (Coming soon)
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Cost per Strain |
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POA |
- Up to 3 sessions of IVF (per strain)
- Embryo transfers into recipients
- Consumables
- Reanimation Report with the following results:
- 2-cell fertilisation rate
- live born offspring
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- Cage costs after 6 weeks of age
- Genotyping
- Health screen
- Shipping costs |
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POA |
- Provision of 3 straws of frozen sperm (for re-animation at the facility
of your choice)
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- Shipment of straws to the facility of your
choice
- Permits (if applicable)
- Management fee |
Embryo Cryopreservation
Requests for Embryo cryopreservation are considered on a case by case basis.
Please see the Embryo Cryopreservation page for more information including animal requirements.
Depositor is eligible for financial support for embryo cryopreservation if:
- All of the basic support criteria above are met and,
- Strain cannot be frozen down as sperm due to loss of genetic integrity e.g. congenic strains.
Support does not cover
- Production of donors, depositor is responsible or the cost of producing the required number of animals of correct sex and age.
- Transportation of animals to cryopreservation facility
- Strain quality assurance
Unrestricted Strains
For strains that are in the public domain, i.e. published, or available to other researchers.
|
Cost per strain |
|
|
|
POA |
- Consumables individually identified with a unique barcode
- Cryopreservation
- Cryopreservation report
- Sample Storage
- Data storage
- Quality Assurance - one validation to live pups
|
- Shipping of animals to the cryopreservation
facility
- Breeding animals for donors (approx cage
cost =$2500) |
Restricted Strains
For those strains that are not freely distributable depositors will be charged for the cryopreservation, on going maintenance (a yearly fee) and for shipment.
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Cost per strain |
Includes |
Excludes |
|
POA |
- Consumables individually identified with a unique barcode
- Cryopreservation of up to 10 sessions
- Data storage
- Cryopreservation report
|
- Breeding animals for donors (approx cage
cost =$2500)
- Shipping of animals to the cryopreservation
facility |
|
POA |
- Duplicate storage at 2 locations |
|
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POA |
- Test thaw and incubation to Blastocyst
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Reanimation from Archived Embryos
Please see the Reanimation Page for more information on the reanimation services offered by the APB. (Coming soon)
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Cost per strain |
Includes |
Excludes |
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POA |
- Up to 3 Thawing sessions
- Embryo transfers into recipients
- Consumables
- Reanimation report
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- Cage costs after 6 weeks of age
- Genotyping
- Health screen
- Shipping costs |
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$400 +GST |
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- Shipment of straws to the facility of your
choice |
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