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Mouse Strain information

APB ID

Added

06/02/2006

Last Edited

11/05/2014

Strain nomenclature:

C57BL/6JSfdAnu-Nfkb2xdr/AnuApb

Nickname:

Xander

Strain designation name:

C57BL/6JSfdAnu-Nfkb2xdr/AnuApb

Genetics

Strain Types

Mutant strain
Coisogenic

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100

Chromosome

Affected Gene Symbol

NFkb2

Protein expression of altered gene:

Nil

Genetic alteration of gene:

ENU induced mutation

Location on chromosome (bp):

46380108-46386580 bp, + strand (From NCBI annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1099800

Synonyms

lyt, NF-kappaB2, p49, p49/p100, p50B, p52

Allele Name

xander

Allele Symbol

Nfkb2xdr

MGI Allele Accession ID

MGI:2667230

Ensembl Gene ID:

ENSMUSG00000025225

Transcript ID:

ENSMUST00000073116

Transcript name:

Nfkb2-201

bp change:

T to A

Intron number:

Genomic position:

46381842

Sequence:
46381812	tcatggtcaaagaggacaaagcctcctcctTgtcctctccagATTTAATAATCTGGGTGTCC	genomic (forward strand)
	Exons

Version:

NCBI m37, Ensembl build 55

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Normal Thy1.2⁺ cells Reduced B220⁺ cells Decrease numbers of peripheral B cells, normal numbers of BM B cells Normal T cell numbers Decreased mature B2 cells in spleen Seven fold decrease in B1 cells in peritoneal cavity Small peripheral lymph nodes Disturbed splenic microarchitecture Lack of follicular dendritic cell clusters in spleen. Intrinsic to B cells

Original Genetic Background

C57BL/6JSfdAnu

Genetic Background Currently Maintained

C57BL/6JSfdAnu

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

Xander (Amplifluor)
Xander (Xdr)

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

B cell
follicular dendritic cell
spleen
lymphocyte

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 2 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

Unknown

MTA needs to be signed?

Yes