Mouse Strain information
APB ID 867
Added 25/05/2007
Last Edited 11/05/2014
Strain name: B6;129P2-Fastm1Osa/Apb
Nickname: B6.Fas-/-
Strain designation name: B6;129P2-Fastm1Osa/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: Fas (TNF receptor superfamily member)  
Chromosome 19
Affected Gene Symbol Fas
Protein expression of altered gene: Nil
Genetic alteration of gene: A BetaGal-neo cassette was used to delete a portion of exon 9, which codes for the cytoplasmic domain essential for Fas-mediated apoptosis. Northern blot analysis revealed the expression of a truncated mRNA, representing transcripts from exons 1 though 8 in the thymus, liver, and heart. Immunoprecipitation analysis showed an absence of the wild-type protein as well as a faint presence of what the authors speculated to be a non-functional Tnfrsf6-BetaGal fusion protein.
Location on chromosome (bp): 34365199-34402260 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:95484
Synonyms APO-1, CD95, TNFR6, Tnfrsf6
Allele Name targeted mutation 1, Osaka University Medical School
Allele Symbol Fastm1Osa
MGI Allele Accession ID MGI:1861923
Allele synonyms: Fas-, Tnfrsf6tm1Osa
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Premature death: 50% dead at about 5 months of age Abnormal hepatocyte morphology: hepatocyte nuclear areas are variable and larger. Liver hyperplasia: * significantly heavier at 12 weeks of age and becomes 50-60% heavier by 16 weeks of age. * number of hepatocytes per each liver lobe is increased immune system. Increased leukocyte cell number: 20x higher than in controls, attributable to increased lymphocyte numbers. Increased lymphocyte cell number: * lymphocytosis; progressively accumulate abnromal T cells Thy1+ B220+ CD4- CD8-. Increased B cell number: increase in the number of normal B cells. Abnormal T cell clonal deletion: * peripheral clonal deletion of mature T cells against a bacterial superantigen is impaired. Abnormal lymph organ size. Enlarged spleen: spleen becomes progressively larger. Enlarged lymph nodes: lymph nodes become progressively larger. Lymphoid hyperplasia. Increased IgG level: 10-100x higher serum levels of IgG1, IgG2a, IgG2b, and IgG3 at 16 weeks of age. Increased IgG1 level. Increased IgG2a level. Increased IgG2b level. Increased IgG3 level. Increased autoantibody level: * 50-500x higher levels of dsDNA and ssDNA autoantibodies at 16 weeks of age. Increased anti-double stranded DNA antibody level. Increased anti-single stranded DNA antibody level. Increased susceptibility to parasitic infection: * decreased survival after infection with Trypanosoma cruzi even though were able to clear parasites from blood and affected organs. Hypersensitivity: * occasional infiltration of lymphocytes into tissues such as the lungs and liver.
Original Genetic Background 129P2/OlaHsd
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
PCR protocols Fas(-/-)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Yes
Description of human condition: Autoimmune Lymphoproliferative Syndrome; ALPS
Applicable Research Areas Virology
Apoptosis
Immunology and inflammation
Keywords
  • autoimmunity
  • hepatocyte
  • lymphocyte
  • T cell
  • B cell
Additional Information Reported this mutant does not express any Fas protein as opposed to spontaneous mutant
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 11 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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