Mouse Strain information
APB ID 86
Added 06/02/2006
Last Edited 10/05/2014
Strain name: C57BL/6JSfdAnu-Lcp2twm/AnuApb
Nickname: T-wimp
Strain designation name: C57BL/6JSfdAnu-Lcp2twm/AnuApb
Genetics
Strain Types Mutant strain
Coisogenic
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: lymphocyte cytosolic protein 2  
Chromosome 11
Affected Gene Symbol Lcp2
Protein expression of altered gene: Reduced
Genetic alteration of gene: ENU induced mutation. A T-to-G transversion in intron 12 that results in altered mRNA splicing. Homozygous mutant mice express two processed transcripts, the normal, wild type mRNA and a second lacking exon 12 and having a premature stop codon in exon 13. T cells of mutant mice express reduced levels of SLP76/LCP2.
Location on chromosome (bp): 33946920-33992295 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1321402
Synonyms SLP-76, SLP76, twm
Allele Name t-wimp
Allele Symbol Lcp2twm
MGI Allele Accession ID MGI:3614800
Ensembl Gene ID: ENSMUSG00000002699
Transcript ID: ENSMUST00000052413
Transcript name: Lcp2-001
bp change: T to G
Intron number: 12
Genomic position: 33978410
Sequence:
33978380tgcagCCCTCAGCCCCATTGGGGAGgtaagTgtctgtcatactgtaccaagtccagaattctgenomic (forward strand)
    Exons
Version: NCBI m37, Ensembl build 55
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Few naïve CD4⁺ and CD8⁺ T cells, autoimmunity, 5 fold decrease in DP Tcells and significantly fewer CD4⁺ and CD8⁺ SP cells, partial block in T cell deveolpment at the Pre TCR checkpoint, very low expression of CD5 on DP thymocytes, defect in TCR signalling, mutation intrinsic to thymocytes, reduced expression of Slp76, Defects in positive selection and reduced negative thymocyte selection, hypergammaglobulinemia and hyper-IgE
Original Genetic Background C57BL/6JSfdAnu
Genetic Background Currently Maintained C57BL/6JSfdAnu
Strain identification
How is this strain characterised? Genotyping
PCR protocols Twimp_(Twp)
Twimp (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • T cell
  • CD8
  • hypergammaglobulinaemia
  • autoimmunity
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 14 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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