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Mouse Strain information

APB ID

Added

06/02/2006

Last Edited

10/05/2014

Strain nomenclature:

C57BL/6JSfdAnu-Lcp2twm/AnuApb

Nickname:

T-wimp

Strain designation name:

C57BL/6JSfdAnu-Lcp2twm/AnuApb

Genetics

Strain Types

Mutant strain
Coisogenic

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: lymphocyte cytosolic protein 2

Chromosome

Affected Gene Symbol

Lcp2

Protein expression of altered gene:

Reduced

Genetic alteration of gene:

ENU induced mutation. A T-to-G transversion in intron 12 that results in altered mRNA splicing. Homozygous mutant mice express two processed transcripts, the normal, wild type mRNA and a second lacking exon 12 and having a premature stop codon in exon 13. T cells of mutant mice express reduced levels of SLP76/LCP2.

Location on chromosome (bp):

33946920-33992295 bp, + strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1321402

Synonyms

SLP-76, SLP76, twm

Allele Name

t-wimp

Allele Symbol

Lcp2twm

MGI Allele Accession ID

MGI:3614800

Ensembl Gene ID:

ENSMUSG00000002699

Transcript ID:

ENSMUST00000052413

Transcript name:

Lcp2-001

bp change:

T to G

Intron number:

Genomic position:

33978410

Sequence:
33978380	tgcagCCCTCAGCCCCATTGGGGAGgtaagTgtctgtcatactgtaccaagtccagaattct	genomic (forward strand)
	Exons

Version:

NCBI m37, Ensembl build 55

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Few naïve CD4⁺ and CD8⁺ T cells, autoimmunity, 5 fold decrease in DP Tcells and significantly fewer CD4⁺ and CD8⁺ SP cells, partial block in T cell deveolpment at the Pre TCR checkpoint, very low expression of CD5 on DP thymocytes, defect in TCR signalling, mutation intrinsic to thymocytes, reduced expression of Slp76, Defects in positive selection and reduced negative thymocyte selection, hypergammaglobulinemia and hyper-IgE

Original Genetic Background

C57BL/6JSfdAnu

Genetic Background Currently Maintained

C57BL/6JSfdAnu

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

Twimp (amplifluor)
Twimp_(Twp)

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

T cell
CD8
hypergammaglobulinaemia
autoimmunity
ENU

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 14 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?

Yes