Mouse Strain information
APB ID 845
Added 23/05/2007
Last Edited 11/05/2014
Strain name: C57BL/6-Ightm1Rbr Tg(IgkHyHEL10)1Rbr/AnuApb
Nickname: SWHEL-HL
Strain designation name: C57BL/6-Ightm1Rbr Tg(IgkHyHEL10)1Rbr/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Transgenic
Mode of Inheritance Dominant
1.  Affected Gene Name: immunoglobulin heavy chain complex  
Chromosome 12
Affected Gene Symbol Igh
Genetic alteration of gene: The HyHEL10 hybridoma produces an antibody that binds hen egg lysozyme peptide (HEL) with high affinity. The rearranged variable heavy gene segment from the HyHEL10 hybridoma replaced the endogenous J gene segments of the IgH locus. A floxed neomycin cassette was placed upstream for targeting purposes. About 0.4% of B cells produce antibody that bind HEL with high affinity.
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:96442
Allele Name immunoglobulin heavy chain complex; targeted mutation 1, Robert Brink
Allele Symbol Ightm1Rbr
MGI Allele Accession ID MGI:3800383
Allele synonyms: VH-10tar
1.   Transgene: transgene insertion 1, Robert Brink  
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State B cells express Immunoglobulin specific for hen egg lysozyme and are capable of undergoing class switch.
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Research tools
Immunology and inflammation
Keywords
  • Immunoglobulin
  • Hen Egg lysozyme (HEL)
  • self-tolerance
  • autoimmunity
  • B cell
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 7 mice)

APB stock genotyped verified by: The APF
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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