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Mouse Strain information

APB ID

Added

06/02/2006

Last Edited

09/05/2014

Strain nomenclature:

C57BL/6JSfdAnu-Hnrnpllthdr/AnuApb

Nickname:

Thunder

Strain designation name:

C57BL/6JSfdAnu-Hnrnpllthdr/AnuApb

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: heterogeneous nuclear ribonucleoprotein L-like

Chromosome

Affected Gene Symbol

Hnrpll

Protein expression of altered gene:

Nil

Genetic alteration of gene:

ENU induced mutation

Location on chromosome (bp):

80429683-80461608 bp, - strand (From Ensembl annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1919942

Allele Name

heterogeneous nuclear ribonucleoprotein L-like; thunder

Allele Symbol

Hnrpllthdr

MGI Allele Accession ID

MGI:3707008

Ensembl Gene ID:

ENSMUSG00000024095

Transcript ID:

ENSMUST00000061331

Transcript name:

Hnrpll-201

bp change:

T to A at position 683 on cDNA

Exon number:

Exon ID:

ENSMUSE00000138333

cDNA position:

683

Amino acid change:

Valine to Aspartic acid at position 136

Sequence:
653	TTGTCCATGTTCGAGGGCTCTGTGAATCTGTTGTGGAAGCTGACCTTGTGGAGGCACTGGA	cDNA (reverse strand)
126	V--V--H--V--R--G--L--C--E--S--V--V--E--A--D--L--V--E--A--L--E	protein

Version:

NCBI m37, Ensembl build 55

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Low T cell numbers which are hyperactivated. By flow cytometric staining of blood, initially detected a strain with CD4 T cells decreased to 25% of wild-type. Further analysis revealed entirely normal thymic development and single positive cells, but a marked reduction in the number of peripheral T cells especially naïve CD4 and CD8 cells, and poor survival of thunder T cells upon adoptive transfer. During this analysis, it was found that thunder thymocytes stain with the RA3-6B2 antibody that selectively recognizes the CD45RABC B220 isoform normally restricted to B cells. Staining with antibodies to CD45RA, B or C showed a 50-fold increase in staining on all thymocytes and peripheral T cells, as well as macrophages and dendritic cells, so that the staining intensity matched that on B cells. Amplification of cDNA with primers spanning CD45 exons 4-6 showed a dramatic increase in the inclusion of these exons in thymocyte CD45 mRNA, establishing that the thunder mutation interferes with the normal CD45 exon 4-6 silencing process.

Original Genetic Background

C57BL/6JSfdAnu

Genetic Background Currently Maintained

C57BL/6JSfdAnu

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

T cell
B cell
lymphocyte
CD45
ENU
NIH

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by:

The APF

Are live mice available?

Unknown

MTA needs to be signed?

Yes