Mouse Strain information
APB ID 78
Added 06/02/2006
Last Edited 09/05/2014
Strain name: C57BL/6JSfdAnu-Hnrnpllthdr/AnuApb
Nickname: Thunder
Strain designation name: C57BL/6JSfdAnu-Hnrnpllthdr/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: heterogeneous nuclear ribonucleoprotein L-like  
Chromosome 17
Affected Gene Symbol Hnrpll
Protein expression of altered gene: Nil
Genetic alteration of gene: ENU induced mutation
Location on chromosome (bp): 80429683-80461608 bp, - strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1919942
Allele Name heterogeneous nuclear ribonucleoprotein L-like; thunder
Allele Symbol Hnrpllthdr
MGI Allele Accession ID MGI:3707008
Ensembl Gene ID: ENSMUSG00000024095
Transcript ID: ENSMUST00000061331
Transcript name: Hnrpll-201
bp change: T to A at position 683 on cDNA
Exon number: 2
Exon ID: ENSMUSE00000138333
cDNA position: 683
Amino acid change: Valine to Aspartic acid at position 136
Sequence:
653TTGTCCATGTTCGAGGGCTCTGTGAATCTGTTGTGGAAGCTGACCTTGTGGAGGCACTGGAcDNA (reverse strand)
126V--V--H--V--R--G--L--C--E--S--V--V--E--A--D--L--V--E--A--L--Eprotein
Version: NCBI m37, Ensembl build 55
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Low T cell numbers which are hyperactivated. By flow cytometric staining of blood, initially detected a strain with CD4 T cells decreased to 25% of wild-type. Further analysis revealed entirely normal thymic development and single positive cells, but a marked reduction in the number of peripheral T cells especially naïve CD4 and CD8 cells, and poor survival of thunder T cells upon adoptive transfer. During this analysis, it was found that thunder thymocytes stain with the RA3-6B2 antibody that selectively recognizes the CD45RABC B220 isoform normally restricted to B cells. Staining with antibodies to CD45RA, B or C showed a 50-fold increase in staining on all thymocytes and peripheral T cells, as well as macrophages and dendritic cells, so that the staining intensity matched that on B cells. Amplification of cDNA with primers spanning CD45 exons 4-6 showed a dramatic increase in the inclusion of these exons in thymocyte CD45 mRNA, establishing that the thunder mutation interferes with the normal CD45 exon 4-6 silencing process.
Original Genetic Background C57BL/6JSfdAnu
Genetic Background Currently Maintained C57BL/6JSfdAnu
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • T cell
  • B cell
  • lymphocyte
  • CD45
  • ENU
  • NIH
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: The APF
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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