Mouse Strain information
APB ID 74
Added 20/04/2004
Last Edited 10/05/2014
Strain name: STOCK H2k2 Fastheo/AnuApb
Nickname: theoden ; ENU4AT:050
Strain designation name: STOCK H2k2 Fastheo/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: Fas (TNF receptor superfamily member 6)  
Chromosome 19
Affected Gene Symbol Fas
Protein expression of altered gene: Nil
Genetic alteration of gene: ENU-induced mutation
Location on chromosome (bp): 34365199-34402260 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:95484
Synonyms APO-1, CD95, TNFR6, Tnfrsf6
Allele Name theoden
Allele Symbol Fastheo
MGI Allele Accession ID MGI:4455060
Ensembl Gene ID: ENSMUSG00000024778
Transcript ID: ENSMUST00000025691
Transcript name: Fas-201
bp change: G to A at position 422 on cDNA
Exon number: 4
Exon ID: ENSMUSE00000144910
cDNA position: 422
Amino acid change: Cysteine to Tyrosine at position 139
Sequence:
392TCTACTGCGATTCTCCTGGCTGTGAACACTGTGTTCGCTGCGCCTCGTGTGAACATGGAACcDNA (forward strand)
129F--Y--C--D--S--P--G--C--E--H--C--V--R--C--A--S--C--E--H--G--Tprotein
Version: NCBI m37, Ensembl build 57
1.  Affected Congenic Gene Name: histocompatibility-2, MHC  
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Antinuclear autoantibodies, lymphadenopathy.
Original Genetic Background C57BL/6JStdAnu x C57BL/10.Cg-H2k
Genetic Background Currently Maintained C57BL/6JStdAnu x C57BL/10.Cg-H2k
Strain identification
How is this strain characterised? Genotyping
PCR protocols Theoden (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Research tools
Keywords
  • B cell
  • T cell
  • lymphadenopathy
  • ENU
  • Wellcome Trust
Additional Information Discovered by Wellcome Trust Strategic Award immunization screen and screen for antinuclear autoantibodies by Edyta Kucharska, Dr Carola Vinuesa, Heather Domaschenz and Dr Lina Tze (JCSMR, ANU). Mutation mapped and identified by Dr Lina Tze and Belinda Whittle (JCSMR, ANU). Project guided by Prof Chris Goodnow.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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