Mouse Strain information
APB ID 694
Added 01/05/2007
Last Edited 11/05/2014
Strain name: 129-Galtm1Wyn/Apb
Nickname: Galn KO
Strain designation name: 129-Galtm1Wyn/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: Galanin  
Chromosome 19
Affected Gene Symbol Gal
Protein expression of altered gene: Nil
Genetic alteration of gene: Exons 1 through 5 were replaced with a neomycin selection cassette. Protein encoded by the targeted locus was undetected by radioimmunoassays of homozygous mutant brain and peripheral tissues.
Location on chromosome (bp): 3409917-3414457 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:95637
Synonyms Galn
Allele Name targeted mutation 1, David Wynick
Allele Symbol Galtm1Wyn
MGI Allele Accession ID MGI:2183172
Allele synonyms: Gal KO, galanin -, GALKO
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State abnormal branching of the mammary ductal tree - reduced ductal branching in the mammary glands of 8 week old virgin females reduced numbers of lactotrophs decreased insulin secretion abnormal lactation - females unable to lactate resulting in death of all pups within 48 hours of birth unless fostered out inability to lactate is absolute for first pregnancies but pup survival improves to 20% after later pregnancies abnormal pituitary secretion - prolactin levels in the hypophysis of randomly cycling females reduced about 40%, plasma levels of prolactin are generally normal levels of both prolactin message and peptide reduced in the pituitary 7 days postpartum, serum prolactin also reduced in serum 7 days postpartum estradiol-17beta induces increased prolactin mRNA but not protein abnormal circulating insulin level - 2-deoxy-glucose has less effect on the initial drop in plasma insulin levels but a causes a greater increase in subsequent insulin levels abnormal adenophysis morphology reduced numbers of lactotrophs hippocampal neuron degeneration - kainic acid injection induces significantly increased CA1 (62.9%) and CA2 (44.8%) apoptosis, staurosporine and glutamate both cause increased cell death of hippocampal neurons in culture decreased sensory neuron number, abnormal dorsal root ganglia morphology - dorsal root ganglia of L4 and L5 are reduced 13% relative to controls small peptidergic neurons preferentially lost short period of apoptosis occurs around 3-4 days of age abnormal nervous system regeneration - nerve regeneration reduced by 30-40% sciatic nerve regeneration after injury incomplete until some time between 4 and 5 weeks whereas complete recovery only takes 2 weeks in controls abnormal object recognition memory - increased exploration of objects placed in a familiar setting failure to discriminate familiar objects in a changed location.
Genetic Background Currently Maintained Sv129
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain Research into lactation defficiencies, hormone regulation
Applicable Research Areas Diabetes
Models for human disease
Cell biology
Research tools
Apoptosis
Developmental biology
Endocrine deficiency
Reproduction
Keywords
  • lactation
  • prolactin
  • hormone
  • mammary gland
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


   

 


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