Mouse Strain information
APB ID 651
Added 25/04/2007
Last Edited 24/04/2015
Strain name: FVB.129S6(B6)-Cyp19a1tm1Esi/Apb
Nickname: Aromatase knockout
Strain designation name: FVB.129S6(B6)-Cyp19a1tm1Esi/Apb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
ES Cell-Line Used KG1
Mode of Inheritance Recessive
1.  Affected Gene Name: cytochrome P450, family 19, subfamily a, polypeptide 1  
Chromosome 9
Affected Gene Symbol Cyp19a1
Protein expression of altered gene: Nil
Genetic alteration of gene: Insertion of a neomycin resistance cassette into exon 9 disrupted the gene. Aromatase activity was not detected in ovaries of 12 week old homozygous mutant mice.
Location on chromosome (bp): 54013744-54041249 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:88587
Synonyms Ar, ArKO, aromatase, Cyp19, Int-5, Int5, p450arom
Allele Name targeted mutation 1, Evan R Simpson
Allele Symbol Cyp19a1tm1Esi
MGI Allele Accession ID MGI:2154536
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Abnormal coat color At 3 weeks of age, both males and females exhibit Dull coat Adipose tissue Abnormal fat pad Females exhibited a 50% to 80% increase in mammary fat pad weight Abnormal gonadal fat pad Males and females exhibited a 50% and 50% to 80% increase in gonadal fat pad weight, respectively endocrine/exocrine glands Abnormal ovary morphology The ovaries of mice on a soy free diet exhibited seminiferous tubule-like structures throughout 80% of ovaries, hemorrhagic cysts, and age dependent increase in collagen deposition of collagen in constrast, animals fed a soy diet did not display Sertoli-like cells in follicles absent corpora lutea impaired ovarian folliculogenesis though ovaries contained numerous follicles with abundant granulosa cells, antrum formation was arrested before ovulation no corpora leutea abnormal prostate morphology increase in combined prostate/urinary bladder weight increased seminal gland weight increased weight of seminal vescicles, putatively due to an increased volume of secretions rather than due to structural changes abnormal seminiferous tubule morphology decrease in the volume of epithelium in contrast to Esr1tm1Ksk homozygotes, no decrease in luminal volume decreased testis weight reduction in weight, 1 year of age homeostasis/metabolism increased circulating follicle stimulating hormone level female FSH levels were elevated 3- to 4-fold relative to wild type females increased circulating luteinizing hormone level female LH levels were elevated 2- to 10-fold relative to wild type females LH levels were also increased in male mice increased circulating testosterone level female testosterone level was increased to ~10 times the level in wild type females male testosterone levels varied though they were in general increased relative to those of control males reproductive system abnormal reproductive system morphology abnormal female reproductive anatomy abnormal ovary morphology the ovaries of mice on a soy free diet exhibited seminiferous tubule-like structures throughout 80% of ovaries, hemorrhagic cysts, and age dependent increase in collagen deposition of collagen in constrast, animals fed a soy diet did not display Sertoli-like cells in follicles absent corpora lutea impaired ovarian folliculogenesis though ovaries contained numerous follicles with abundant granulosa cells, antrum formation was arrested before ovulation no corpora leutea abnormal uterus development underdeveloped uterus observed at 12 to 14 weeks of age 3-fold enlargement of the clitoral gland relative to that of wild type abnormal labium morphology labial folds do not develop abnormal spermiogenesis normal at 4.5 months of age dysfunction evident at 1 year of age spermatids did not complete elongation and spermiation degenerating round spermatids abnormal prostate morphology increase in combined prostate/urinary bladder weight increased seminal gland weight increased weight of seminal vescicles, putatively due to an increased volume of secretions rather than due to structural changes abnormal seminiferous tubule morphology decrease in the volume of epithelium in contrast to Esr1tm1Ksk homozygotes, no decrease in luminal volume decreased testis weight reduction in weight, 1 year of age abnormal primary sex determination on a soy free diet, the ovaries develop cells that possess structural and functional characteristics of testicular interstitial (Leydig) cells and of seminiferous tubule-like structures lined with Sertoli cells the development of testicular tissue was postponed and reduced with estrogen replacement female infertility male infertility progressive infertility developing between 4.5 months and 1 year of age
Original Genetic Background 129 x C57BL/6J
Genetic Background Currently Maintained FVB/N
Coat Colour White
Strain identification
How is this strain characterised? Genotyping
PCR protocols Aromatase KO
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Cell biology
Developmental biology
Reproduction
Keywords
  • Cyp19
  • reproductive system
  • adipose
  • cholesterol
  • sperm
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 9 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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