Mouse Strain information
APB ID 641
Added 25/04/2007
Last Edited 11/05/2014
Strain name: STOCK Cbltm1Wlan/Apb
Nickname: c-Cbl (G304E)
Strain designation name: STOCK Cbltm1Wlan/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockin
ES Cell-Line Used W9.5
Mode of Inheritance Recessive
1.  Affected Gene Name: Casitas B-lineage lymphoma  
Chromosome 9
Affected Gene Symbol Cbl
Protein expression of altered gene: Normal
Genetic alteration of gene: A transition point mutation resulted in a glycine to glutamic acid substitution at position 304 (G304E). The substitution is within the tyrosine kinase binding domain (TKB). Targeting also introduced a HSVTK/pGKNeo cassette upstream of the mutation, but the cassette was subsequently removed by Cre-LoxP deletion in vivo.
Location on chromosome (bp): 43957345-44042129 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:88279
Synonyms 4732447J05Rik, c-Cbl, cbl, Cbl-2
Allele Name targeted mutation 1, Wallace Langdon
Allele Symbol Cbltm1Wlan
MGI Allele Accession ID MGI:2451336
Allele synonyms: E
Mutant Construction Technique CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State Mice have a physically normal outward appearance and are generally healthy. Up to ~40% homozygous females have blockages in the uterine horns and fallopian tubes, while ~25% of homozygous males appear infertile. All homozygous mice show slight splenomegaly (~50% larger than wildtype control).
Phenotype Heterozygous State Appear normal and healthy
Original Genetic Background 129S1/SvJ x C57BL/6J
Genetic Background Currently Maintained 129S1/SvJ x C57BL/6J
Coat Colour Agouti
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols c-Cbl G304E genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Cell biology
Immunology and inflammation
Reproduction
Keywords
  • Cbl
  • tyrosine kinase binding domain
  • TKB
  • Zap-70
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


   

 


Build: 2017.09.11 Non-profit supported by the Australian government  |  © Copyright 2017 Australian Phenomics Facility Last updated: 22/09/2017