Mouse Strain information
APB ID 640
Added 25/04/2007
Last Edited 15/06/2014
Strain name: C.B6-Gnaztm1Iah/AnuApb
Nickname: Gz alpha knockout
Strain designation name: C.B6-Gnaztm1Iah/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Dominant
1.  Affected Gene Name: guanine nucleotide binding protein, alpha z subunit  
Chromosome 10
Affected Gene Symbol Gnaz
Protein expression of altered gene: Nil
Genetic alteration of gene: The alpha subunit was ablated by the insertion of a PGK-neo cassette 160 bp downstream of the translation start site. Alpha subunit protein was absent in Western blots of brains and other tissues of mutant mice.
Location on chromosome (bp): 74429922-74479652 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:95780
Synonyms Gz
Allele Name targeted mutation 1, I A Hendry
Allele Symbol Gnaztm1Iah
MGI Allele Accession ID MGI:3575007
Allele synonyms: Gzalpha-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Hypertolerant to morphine, show anxiety. In previously unexposed female homozygotes reduced analgesia at low morphine doses is seen, a similar but not significant effect was seen in males. With repeated morphine doses mutants develop tolerance faster than wild type mice. Morphine treated homozygotes are able to tolerate higher morphine doses compared to wild type mice (LD50 of 800 mg/kg compared to 700 mg/kg for wild type) however no significant difference in the serum levels of morphine or its metabolites is seen reproductive system Litters from homozygous parents on the C57BL/6 background had increased mortality that was not seen in litters from heterozygous or mixed strain parents touch/vibrissae.
Phenotype Heterozygous State Decreased sensitivity to xenobiotic induced morbidity/mortality: morphine treated homozygotes are able to tolerate higher morphine doses compared to wild-type mice (LD50 of 750 mg/kg compared to 700 mg/kg for wild-type). Abnormal chemically-elicited antinociception: with repeated morphine doses heterozygotes develop tolerance faster than wild-type mice but not as quickly as homozygous mutants.
Original Genetic Background C57BL/6
Genetic Background Currently Maintained BALB/c
Coat Colour White
Eye Colour Red
Strain identification
How is this strain characterised? Genotyping
PCR protocols GαZ KO
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain Increased tolerance to opiates
Applicable Research Areas Models for human disease
Sensorineural
Neurobiology
Cell biology
Developmental biology
Research tools
Keywords
  • morphine
  • receptor
  • G protein
  • tolerance
  • feeding behaviour
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 6 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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