Mouse Strain information
APB ID 631
Added 25/04/2007
Last Edited 11/05/2014
Strain name: B6;129-Eif2ak2tm1Cwe/Apb
Nickname: Pkr KO
Strain designation name: B6;129-Eif2ak2tm1Cwe/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
ES Cell-Line Used GS1
Mode of Inheritance Recessive
1.  Affected Gene Name: eukaryotic translation initiation factor 2-alpha kinase 2  
Chromosome 17
Affected Gene Symbol Eif2ak2
Protein expression of altered gene: Nil
Genetic alteration of gene: A 2.0kb region, containing portions of exons 2 and 3, was deleted by the insertion of a neomycin selection cassette adjoined to the 5' end of an upstream mouse sequence (UMS) element. Northern blot analyses, using various probes, showed that transcription was not terminated by the UMS element. RT-PCR and sequence analysis showed that exons 2 and 3 and the intervening neo cassette had been spliced out in the majority of transcripts. The exon-skipped transcript was shown to be capable of producing protein in a cell-free system, however, protein was undetected via Western blot analysis of lysates obtained from homozygous mutant mice. Additionally, assays of MEF cell extracts showed an absence of autophosphorylation activity in homozygous mutant mice.
Location on chromosome (bp): 79251904-79281912 bp, - strand (From NCBI annotation of NCBI Build 36)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1353449
Synonyms dsRNA-activated kinase, eIF-2 alpha, eIF-2 alpha, Eif2ak2, IFN- type I-induced and dsRNA-activated kinase, IFN-induced and double-stranded RNA-activated kinase, Pkr, Prkr, Tik
Allele Name targeted mutation 1, Charles Weissmann
Allele Symbol Eif2ak2tm1Cwe
MGI Allele Accession ID MGI:2182566
Allele synonyms: N-PKR-, PKR-, Pkro
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Mice homozygous for disruptions in this gene display altered susceptibility to viral infection. Enlarged lymph nodes. Altered susceptibility to viral infection. increased T cell proliferation. Proliferative responses of both CD4+ and CD8+ T-cells from sensitized mice is somewhat increased abnormal inflammatory mediator physiology. A 2 fold increase in Il4 production by CD44+ cells increased susceptibility to viral infection. Susceptibility to encephalomyocarditis virus tested response to infection and survival were similar to control mice; however, pretreatment of mice with IFNgamma failed to improve survival as it did in controls increased susceptibility to type IV hypersensitivity reaction. Exaggerated response to challenge after presensitization in contact hypersensitivity test effect can be transferred to naive mice using CD8+ cells from sensitized mice.
Phenotype Heterozygous State Normal
Original Genetic Background 129Sv x C57BL/6
Genetic Background Currently Maintained C57BL/6
Coat Colour Black / Agouti
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols RKO
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Research value of this Strain Study of Innate immunity
Applicable Research Areas Immunology and inflammation
Keywords
  • T cell
  • stimulation
  • viral infection
  • hypersensitivity
  • proliferation
Additional Information Control strain also frozen down, APB ID#4712
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


   

 


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