Mouse Strain information
APB ID 630
Added 25/04/2007
Last Edited 11/05/2014
Strain name: B6;129S1-Tlr3tm1Flv/Apb
Nickname: T3RKO
Strain designation name: B6;129S1-Tlr3tm1Flv/Apb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
ES Cell-Line Used W9.5/W95
Mode of Inheritance Recessive
1.  Affected Gene Name: toll-like receptor 3  
Chromosome 8
Affected Gene Symbol Tlr3
Protein expression of altered gene: Normal
Genetic alteration of gene: The gene was disrupted by replacement of exon 1 with a floxed neo cassette via homologous recombination. Northern blot analysis detected a truncated transcript in mutant animals. The mutant transcript does not encode a functional protein.
Location on chromosome (bp): 46481019-46495893 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:2156367
Allele Name targeted mutation 1, Richard A Flavell
Allele Symbol Tlr3tm1Flv
MGI Allele Accession ID MGI:2653138
Allele synonyms: TLR-, Tlr3-
Mutant Construction Technique CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State Lymph node enlargement. Normal lymphocyte development. Normal expression of CD3, B220, CD4, and CD8 in thymocytes and splenocytes Abnormal B cell physiology. Impaired expression of CD69, CD80, and CD86 in response to poly(I:C), but not LPS Impaired expression of CD69 in response to viral genomic dsRNA from Type I Lang mammalian reovirus Abnormal macrophage physiology. Impaired ability to produce IL6, IL12 and TNF-alpha in response to poly(I:C), a synthetic dsRNA analogue production of IL6, IL12, and TNF-alpha in response to LPS, PGN, LTA, zymosan, mannan, or CpG DNA was comparable to wild type controls Decreased inflammatory response. Resistant to poly(I:C)-induced shock compared to wild type mice produced less IL12 after poly(I:C) i.p. injection. Upregulation of costimulatory molecules, CD40 and CD86, by peritoneal macrophages in response to dsRNA was preserved on C57BL/6 background. Impaired ability to produce type I interferon and TNF-alpha in response to dsRNA, but not in response to LPS.
Phenotype Heterozygous State Normal
Original Genetic Background 129SV x C57BL/6
Genetic Background Currently Maintained C57BL/6
Coat Colour Black / Agouti
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Research value of this Strain For the Study of innate immunity
Applicable Research Areas Immunology and inflammation
Keywords
  • B cell
  • lymphocyte
  • Macrophage
  • inflammatory response
  • LPS
Additional Information Control strain also frozen down
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? No


   

 


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