Mouse Strain information
APB ID 629
Added 25/04/2007
Last Edited 11/05/2014
Strain name: B6;129S4-Elf5tm1Mapr/Apb
Nickname: Elf5 KO
Strain designation name: B6;129S4-Elf5tm1Mapr/Apb
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Targeted knockout
ES Cell-Line Used J1
Mode of Inheritance Semi-dominant
1.  Affected Gene Name: E74-like factor 5  
Chromosome 2
Affected Gene Symbol Elf5
Protein expression of altered gene: Nil
Genetic alteration of gene: Part of exon 3 was replaced with an NLS-LacZ-neo cassette. The construct was designed such that a fusion product containing the first 28 amino acids would be fused to Beta-gal. Northern blot of mutant mammary glands indicated that the null allele did not create any alternatively spliced transcripts.
Location on chromosome (bp): 103251870-103291146 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1335079
Synonyms ESE-5
Allele Name targeted mutation 1, Melanie A Pritchard
Allele Symbol Elf5tm1Mapr
MGI Allele Accession ID MGI:3573855
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygous embryos die between E3.5 and E7.5
Phenotype Heterozygous State Background Sensitivity: all heterozygous females resulting from backcrosses to 129T2/SvEms failed to lactate even after multiple pregnancies. Initial development of the mammary gland is normal however development stalls during the proliferation phase resulting in retarded alveolar genesis. About 84% of heterozygous females fail to support any pups and about 6% were able to support a reduced number of pups. No overt phenotype, mammary gland defect in females upon pregnancy resulting in a failure to nurse offspring.
Original Genetic Background 129Sv x C57BL/6
Genetic Background Currently Maintained 129Sv
Coat Colour Agouti
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain Cancer Research - Elf5 has been shown to be dysregulated in human cancer. Pregnancy associated development of the mammary gland - Elf5 controls development of the mammary gland upon pregnancy.
Applicable Research Areas Cell biology
Cancer
Developmental biology
Reproduction
Keywords
  • Mammary gland defect
  • embryonic lethal
  • prolactin receptor
  • pregnancy
Is the strain available in any form to other researchers No
APB stock stored as: Cryopreserved sperm (from 7 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


   

 


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