Mouse Strain information
APB ID 5070
Added 25/03/2010
Last Edited 10/05/2014
Strain name: C57BL/6J-Flt3m1Btlr/BtlrAnuApb
Nickname: Warmflash
Strain designation name: C57BL/6J-Flt3m1Btlr/BtlrAnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: FMS-like tyrosine kinase 3  
Chromosome 5
Affected Gene Symbol Flt3
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU-induced mutation
Location on chromosome (bp): 148142317-148212065 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:95559
Synonyms CD135, Flk-2, Flk2, Flt-3, wmfl
Allele Name FMS-like tyrosine kinase 3; mutation 1, Bruce Beutler
Allele Symbol Flt3m1Btlr
MGI Allele Accession ID MGI:3628823
Allele synonyms: Flt3wmfl, warmflash
Ensembl Gene ID: ENSMUSG00000042817
Transcript ID: ENSMUST00000049324
Transcript name: Flt3-001
bp change: G to A
Intron number: 8
Genomic position: 147367088
Sequence:
147367118aatatctaatttgtaaatttcctttttacaGAAAAAGGGTTTATAAACGCTACCAGCTCGCAgenomic (reverse strand)
    Exons
Version: NCBI m38, Ensembl build 75
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Susceptible to Mouse cytomegalovirus (MCMV) at a dose of 1x105 PFU. Decreased dendritic cell number: conventional DCs are reduced in bone marrow and spleen. Decreased plasmacytoid dendritic cell number: both the number and percentage of pDCs are reduced in bone marrow and spleen. Decreased NK cell number: splenic NK cells are reduced in percentage and number, bone marrow NK cells are only moderately reduced. Decreased splenocyte number: spleens and lymph nodes are consistently smaller and show reduced cellularity. Small lymph nodes. Abnormal dendritic, marcophage and NK cell physiology. Decreased body weight.
Original Genetic Background C57BL/6J
Genetic Background Currently Maintained C57BL/6J
Strain identification
How is this strain characterised? Genotyping
Phenotyping
PCR protocols Warmflash (Amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? Unknown
Does it Model a Human Condition? Unknown
Applicable Research Areas Haematology
Immunology and inflammation
Keywords
  • infection
  • MCMV
  • ENU
  • dendritic cell
  • marcophage
  • NK cell
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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