Mouse Strain information
APB ID 5069
Added 25/03/2010
Last Edited 19/05/2014
Strain name: C57BL/6J-Slfn2m1Btlr/BltrAnuApb
Nickname: Elektra
Strain designation name: C57BL/6J-Slfn2m1Btlr/BltrAnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: schlafen 2  
Chromosome 11
Affected Gene Symbol Slfn2
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU-induced mutation
Location on chromosome (bp): 82878614-82884180 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1313258
Synonyms Shlf2
Allele Name schlafen 2; mutation 1, Bruce Beutler
Allele Symbol Slfn2m1Btlr
MGI Allele Accession ID MGI:3850348
Allele synonyms: elektra, Slfn2eka
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State CD8 deficiency, decreased CD8 response, hematopoietic system, immune system, mouse cytomegalovirus (MCMV) susceptibility, NKT cell deficiency.Elektra mice died six to eight days after infection with 2x10 5 PFU of MCMV, whereas nearly all wild type mice survived.Elektra homozygotes were also highly susceptible to infection with Listeria monocytogenes, exhibiting increased bacterial loads in the liver 2 days post-infection relative to those of wild type mice. Elektra mice displayed normal cellularity of the spleen, thymus, lymph nodes, and peripheral blood. B cells from elektra mice proliferated normally in response to BCR stimulation or CpG treatment. However, antibody production in response to MCMV infection was delayed. Elektra mice lacked NKT (CD3+NK1.1+) cells in the thymus and spleen, and had reduced numbers in the liver. When elektra NKT cells were stimulated with α-galactosylceramide tetramer, they produced greatly reduced levels of IFN-γ and IL-4 compared to wild type cells. Elektra mice displayed a 50% reduction in Foxp3+ regulatory T cells.Elektra CD8+ T cells failed to proliferate upon stimulation with CD3ε and CD28 antibodies, with PMA and ionomycin, or with IL-12 in vitro.
Original Genetic Background C57BL/6J
Genetic Background Currently Maintained C57BL/6J
Strain identification
How is this strain characterised? Genotyping
PCR protocols Elektra (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
General information
Associated IP rights? Unknown
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • CD8
  • MCMV
  • interferon
  • T cell
  • infection
  • NKT
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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