Mouse Strain information
Added 28/11/2005
Last Edited 17/04/2014
Strain name: C57BL/6JSfdAnu-Jak3bfy/AnuApb
Nickname: Buffy
Strain designation name: C57BL/6JSfdAnu-Jak3bfy/AnuApb
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: Janus kinase 3  
Chromosome 8
Affected Gene Symbol Jak3
Protein expression of altered gene: Normal
Genetic alteration of gene: ENU induced mutation
Location on chromosome (bp): 71676296-71690575 bp, + strand From VEGA annotation of GRCm38
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

Allele Name buffy
Allele Symbol Jak3bfy
MGI Allele Accession ID MGI:3611885
Ensembl Gene ID: ENSMUSG00000031805
Transcript ID: ENSMUST00000051995
Transcript name: Jak3-001
bp change: T to C at position 1490 on cDNA
Exon number: 9
Exon ID: ENSMUSE00000329342
cDNA position: 1490
Amino acid change: Leucine to Proline at position 398
Version: NCBI m37, Ensembl build 55
Mutant Construction Technique None
Phenotype Homozygous State Pan-Lymphopenia with hyperactivated CD4⁺ cells Obstructed B cell maturation Reduced B cell numbers in periphery B220⁺CD19⁺cells, IgM⁺IgD⁺ cells or CD21⁺IgD⁺ cells were reduced in the spleen by factor of 3. Peritoneal cavity: reduced IgM⁺IgD⁻ cells and only partially reduced IgM⁺IgD⁺ cell numbers Reduced numbers of thymocytes at all stages of T cell maturation. Thymi are smaller than aged-matched controls. 50% of CD4⁺ cells compared to controls 4 fold lower levels of CD8⁺ cells compared to controls Mutation intrinsic to B and T cells, exaggerated under competitive reconstitution with wild-type cells, SCID mouse. Develop diarrhoea
Original Genetic Background C57BL/6JSfdAnu
Genetic Background Currently Maintained C57BL/6JSfdAnu
Coat Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols Buffy (Buf)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
  • T cell
  • B cell
  • IL-2
  • Thymus
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: The APF
Are live mice available? No
MTA needs to be signed? Yes



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