Home - Australian Phenomics Facility

Mouse Strain information

APB ID

4998

Added

28/01/2010

Last Edited

10/05/2014

Strain nomenclature:

C57BL/6JAnu-Cdk4wnch/AnuApb

Nickname:

Wanchi ; ENU8B6:053

Strain designation name:

C57BL/6JAnu-Cdk4wnch/AnuApb

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: cyclin-dependent kinase 4

Chromosome

Affected Gene Symbol

Cdk4

Genetic alteration of gene:

ENU-induced mutation. T to A substitution in the Stop codon (TGA) of the gene in exon 8. This results in a STOP to a Arg change. There will be an additional 40 aa added to the end of the protein before another STOP.

Location on chromosome (bp):

126500590-126504976 bp, + strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:88357

Synonyms

Crk3, p34PSK-J3/cdk4

Allele Name

Wanchi

Allele Symbol

Cdk4wnch

MGI Allele Accession ID

MGI:5009264

Ensembl Gene ID:

ENSMUSG00000006728

Transcript ID:

ENSMUST00000006911

Transcript name:

Cdk4-001

bp change:

T to A at position 1152 on cDNA

Exon number:

Exon ID:

ENSMUSE00000796826

cDNA position:

1152

Amino acid change:

STOP to Arginine at position 304

Sequence:
1122	TACCTGCACAAGGAGGAAAGCGACGCAGAGTGAGAAGAGGGGCTGCCTTTCCCAGTCTTGG	cDNA (forward strand)
294	-Y--L--H--K--E--E--S--D--A--E--*-..............................	protein

Version:

NCBI m37, Ensembl build 57

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Diabetic as measured by glucose - diastix test. Diabetes independent of el/TCR transgenes.

Original Genetic Background

C57BL/6

Genetic Background Currently Maintained

C57BL/6

Strain identification

How is this strain characterised?

Phenotyping
Genotyping

PCR protocols

Wanchi

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation
Diabetes

Keywords

T cell
glucose
ENU
Wellcome Trust
JDRF

Additional Information

Discovered by Wellcome Trust Strategic Award. Identified in immunization antibody response screen by Ms Edyta Kucharska and Dr Anselm Enders (JCSMR, ANU). Project guided by Prof Chris Goodnow.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 4 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

Unknown

MTA needs to be signed?

Yes