Mouse Strain information
APB ID 4970
Added 15/01/2010
Last Edited 19/05/2014
Strain name: B6;C-Fliitm1Hdc/AnuApb
Nickname: B6.Flii 1.5F
Strain designation name: B6;C-Fliitm1Hdc/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
ES Cell-Line Used BALB/c-I
Mode of Inheritance Recessive
1.  Affected Gene Name: flightless I homolog (Drosophila)  
Chromosome 11
Affected Gene Symbol Flii
Protein expression of altered gene: Nil
Genetic alteration of gene: Portions of exons 4 and 5 and all of intron 4 are replaced with an in-frame neo-resistance gene. Cryptic splicing around the insertion will result in a frameshift in the coding region.
Location on chromosome (bp): 60527625-60540765 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1342286
Synonyms Fli1, Fliih
Allele Name targeted mutation 1, Hugh D Campbell
Allele Symbol Fliitm1Hdc
MGI Allele Accession ID MGI:2179825
Allele synonyms: FliI-, Fliih-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Embryonic lethality at implantation: no embryos are recovered after E5.5; embryos develop normally until cellularization, the development arrests before completion of gastrulation blastocysts initiate uterine implantation but embryos fail to develop. embryonic growth arrest: development arrests during gastrulation
Phenotype Heterozygous State Enhanced wound healing: 3 days after a 1 cm full-thickness incision was made in the dorsal skin, surface wound area measurements show smaller, more contracted wounds than in wild-type mice. Length of neoepidermis is significantly greater in mutant animals, and percentage or re-epithelialization of wounds is greater at 5 days after wounding. By day 7, healing is comparable in mutants and wild-type
Original Genetic Background BALB/c
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
PCR protocols Flii Knockout
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Yes
Applicable Research Areas Dermatology
Developmental biology
Keywords
  • cell migration
  • embryonic lethal
  • wound repair
  • gelsolin
  • gastrulation
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 4 mice)

APB stock genotyped verified by: The depositor
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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