Home - Australian Phenomics Facility

Mouse Strain information

APB ID

4839

Added

24/09/2009

Last Edited

10/05/2014

Strain nomenclature:

STOCK Muc2keny/AnuApb

Nickname:

Kenny ; ENU9CAT:040

Strain designation name:

STOCK Muc2keny/AnuApb

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: mucin 2

Chromosome

Affected Gene Symbol

Muc2

Protein expression of altered gene:

Unknown

Genetic alteration of gene:

ENU-induced mutant

Location on chromosome (bp):

148930725-148940442 bp, + strand (From NCBI annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1339364

Allele Name

kenny

Allele Symbol

Muc2keny

MGI Allele Accession ID

MGI:4820425

Ensembl Gene ID:

ENSMUSG00000025515

Transcript ID:

ENSMUST00000026590

Transcript name:

Muc2-202

bp change:

C to A at position 4192 on cDNA

Exon number:

Exon ID:

ENSMUSE00000519387

cDNA position:

4192

Amino acid change:

Serine to STOP at position 1396

Sequence:
4162	CAATGGAATACTGTACTCCTTCAACTATCTCACCTACAACTTCAACAACAACCTTGTCTAC	cDNA (forward strand)
1386	P--M--E--Y--C--T--P--S--T--I--S--P--T--T--S--T--T--T--L--S--T	protein

Version:

NCBI m37, Ensembl build 55

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Diarrhoea. Multiple plaques on GIT and lymphomegaly. Other mutants homozygote for a point mutation have soft feces at weaning and develop diarrhea associated with malapsorption syndrome. Homozygous null mutants pass blood in their feces at 6 months, and 65% of null mutants have intestinal tumors at 1 year.

Original Genetic Background

C57BL/6 x B10.BR x CBA

Genetic Background Currently Maintained

C57BL/6 x CB10.BR x CBA

Coat Colour

Black

Eye Colour

Black

Strain identification

How is this strain characterised?

Phenotyping

Phenotyping technique:

Visual appearance

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Yes	Yes
Are homozygous mice fertile?	Yes	Yes
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Homozygous Matings Required?	No
Development Summary	Cross of ins-HEL transgenic and 3A9 (TCR) transgenic mice. C57BL/6 mles were treated with ENU. Once fertility was recovered the ENU treated males were mated with female insHEL:3A9 mice. Once potential mutants identified mapping crosses were established using CBA background mice.
Male Fertility Compromised?	No
Breeding Performance	Unknown

Relevant bibliographic / database references

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Developmental biology
Gastrointestinal
Immunology and inflammation

Keywords

malabsorption
colon
goblet cells
diarrhoea
ENU

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?

Yes