Home - Australian Phenomics Facility

Mouse Strain information

APB ID

Added

28/04/2004

Last Edited

15/06/2014

Strain nomenclature:

C57BL/6JSfdAnu-Cd79am1Anu/AnuApb

Nickname:

Kenobi ; ENU5strain:045; ENU5WT:054

Strain designation name:

C57BL/6JSfdAnu-Cd79am1Anu/AnuApb

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: CD79A antigen (immunoglobulin-associated alpha)

Chromosome

Affected Gene Symbol

Cd79a

Protein expression of altered gene:

Nil

Genetic alteration of gene:

ENU induced mutation

Location on chromosome (bp):

25682400-25687216 bp, + strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:101774

Synonyms

Cd79a, Ig alpha, Ig-alpha, Iga, Ly54, mb-1

Allele Name

CD79A antigen (immunoglobulin-associated alpha); mutation 1, The Australian National University

Allele Symbol

Cd79am1Anu

MGI Allele Accession ID

MGI:5006856

Allele synonyms:

Cd79aken, ENU5WT:054, Kenobi

Ensembl Gene ID:

ENSMUSG00000003379

Transcript ID:

ENSMUST00000003469

Transcript name:

Cd79a-001

bp change:

G to A at position 327 on cDNA

Exon number:

Exon ID:

ENSMUSE00000199568

cDNA position:

327

Amino acid change:

Tryptophan to STOP at position 62

Sequence:
297	CAATGGCAGGAACCCTAATATCACATGGTGGTTCAGCCTTCAGTCTAACATCACATGGCCC	cDNA (forward strand)
52	--N--G--R--N--P--N--I--T--W--W--F--S--L--Q--S--N--I--T--W--P-	protein

Version:

NCBI m37, Ensembl build 62

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Immune deficiency. Hypogammaglobulinemia. Absolutely no B cells or pre-B cells.

Original Genetic Background

C57BL/6

Genetic Background Currently Maintained

C57BL/6 x C57BL/10.Br

Strain Images

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

KENOBI (amplifluor)

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation
Research tools

Keywords

B-cell antigen receptor
B cell
hypogammaglobulinemia
ENU
Wellcome Trust

Additional Information

Discovered by Wellcome Trust flow cytometry and immunization screens by Sara Dawson, Edyta Kucharska and Dr Lina Tze (JCSMR, ANU). Project guided by Prof Chris Goodnow. Looks like no one kept this strain before crossing to other strains.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 7 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?

Yes