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Mouse Strain information

APB ID

4512

Added

26/02/2009

Last Edited

10/05/2014

Strain nomenclature:

C57BL/6JSfdAnu-Pnpbata/AnuApb

Nickname:

bata ;ENU8B6:058

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: purine-nucleoside phosphorylase

Chromosome

Affected Gene Symbol

Pnp

Genetic alteration of gene:

ENU-induced mutation.

Location on chromosome (bp):

51563977-51573087 bp, + strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97365

Synonyms

Np, Np-1, Np-2, Pnp, Pnp1

Allele Name

bata

Allele Symbol

Pnpbata

MGI Allele Accession ID

MGI:5468722

Ensembl Gene ID:

ENSMUSG00000021871

Transcript ID:

ENSMUST00000048615

Transcript name:

Pnp-001

bp change:

A to T at position 925 on cDNA

Exon number:

Exon ID:

ENSMUSE00000617134

cDNA position:

925

Amino acid change:

Aspartic acid to Valine at position 215

Sequence:
895	AGAGTCGTCTGCTAAAGATGCTGGGGGCAGATGCTGTTGGCATGAGCACAGTCCCAGAAGT	cDNA (forward strand)
205	E--S--R--L--L--K--M--L--G--A--D--A--V--G--M--S--T--V--P--E--V	protein

Version:

NCBI m37, Ensembl build 62

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Decreased anti-IgM B cell proliferation in vitro, age-dependent increase in CD44hi ICOShi CD4 cells and decrease in marginal zone B cells.

Original Genetic Background

C57BL/6JSfdAnu

Genetic Background Currently Maintained

C57BL/6JSfdAnu

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

B cell
T cell
ENU
Wellcome Trust

Additional Information

Discovered by NIH B cell proliferation screen and Wellcome Trust flow cytometry screens by Dr Lina Tze (JCSMR, ANU). Mapped and mutation identified by Dr Lina Tze and Belinda Whittle. Project guided by Prof Chris Goodnow.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 9 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?

Yes