Mouse Strain information
APB ID 4511
Added 26/02/2009
Last Edited 11/05/2014
Strain name: C57BL/10JAnu-Prdx1flro/AnuApb
Nickname: fluoro ; ENU8B6:058
Genetics
Strain Types Mutant strain
Genetic Details Spontaneous
Mode of Inheritance Recessive
1.  Affected Gene Name: peroxiredoxin 1  
Chromosome 4
Affected Gene Symbol Prdx1
Protein expression of altered gene: Unknown
Genetic alteration of gene: Spontaneous mutation, missense
Location on chromosome (bp): 116358149-116373427 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:99523
Synonyms macrophage 23kDa stress protein, macrophase stress protein 22kDa, MSP23, OSF-3, osteoblast specific factor 3, PAG, Paga, Prx I, PrxI, Tdpx2, TDX2, thioredoxin dependent peroxide reductase 2, Trx dependent peroxide reductase 2
Allele Name fluoro
Allele Symbol Prdx1flro
MGI Allele Accession ID MGI:4819263
Ensembl Gene ID: ENSMUSG00000005161
Transcript ID: ENSMUST00000005292
Transcript name: Prdx2-001
bp change: C to G at position 301 on cDNA
Exon number: 3
Exon ID: ENSMUSE00000499125
cDNA position: 301
Amino acid change: Serine to Cysteine at position 79
Sequence:
271GCTGCGAGGTGCTGGGAGTGTCTGTGGACTCTCAGTTCACCCACCTGGCGTGGATCAATACcDNA (forward strand)
69G--C--E--V--L--G--V--S--V--D--S--Q--F--T--H--L--A--W--I--N--Tprotein
Version: NCBI m37, Ensembl build 56
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Autofluorescence, multiple wavelengths, in flow cytometry of all lymphocyte subsets and erythrocytes.
Original Genetic Background C57BL/10
Genetic Background Currently Maintained C57BL/10
Strain Images
Flow cytometry plot of cells from affected animals. No antibodies added.
Flow cytometry plot of cells from affected animals. No antibodies added.
Flow cytometry plot of cells from affected animals. No antibodies added.
Flow cytometry plot of cells from affected animals. No antibodies added.
Strain identification
How is this strain characterised? Genotyping
PCR protocols Fluoro
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Haematology
Keywords
  • lymphocyte
  • erythrocyte, red blood cell
  • ENU
  • Wellcome Trust
Additional Information Discovered by NIH B cell proliferation screen and Wellcome Trust flow cytometry screens by Dr Lina Tze and Dr Katrina Randall (JCSMR, ANU). Mapped and mutation identified by Dr Katrina Randall and Belinda Whittle. Project guided by Prof Chris Goodnow.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 11 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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