Mouse Strain information
APB ID 4498
Added 25/02/2009
Last Edited 10/05/2014
Strain nomenclature: C57BL/6JAnu-Dock8pri/AnuApb
Nickname: primurus, ENU7B6:079
Strain designation name: C57BL/6JAnu-Dock8pri/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: dedicator of cytokinesis 8  
Chromosome 19
Affected Gene Symbol Dock8
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU-induced mutation
Location on chromosome (bp): 25074019-25276922 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:1921396
Allele Name primurus
Allele Symbol Dock8pri
MGI Allele Accession ID MGI:3835421
Ensembl Gene ID: ENSMUSG00000052085
Transcript ID: ENSMUST00000025831
Transcript name: Dock8-201
bp change: T to C at position 5601 on cDNA
Exon number: 42
Exon ID: ENSMUSE00000145941
cDNA position: 5601
Amino acid change: Serine to Proline at position 1827
Sequence:
5571GAACCCGCAATCACGAAGCTCCCGGAGATCTCACATAGACTAGAGGGATTTTATGGCCAGTcDNA (forward strand)
1817-E--P--A--I--T--K--L--P--E--I--S--H--R--L--E--G--F--Y--G--Q--protein
Version: NCBI m37, Ensembl build 57
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Defective antibody response to immunization: failure to sustain antibody formation, undergo affinity maturation or to mount heightened recall (memory) response upon booster immunization. DOCK8-mutant B cells were unable to form marginal zone B cells or to persist in germinal centers and undergo affinity maturation. Dock8 mutations disrupted accumulation of the integrin ligand ICAM-1 in the B cell immunological synapse but did not alter other aspects of B cell antigen receptor signaling.
Original Genetic Background C57BL/6JAnu
Genetic Background Currently Maintained C57BL/6JAnu
Coat Colour Black
Strain Images
primurus homozygous mice (pri/pri), and heterozygous (pri/+) or wild-type (+/+) siblings were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS), and heat-killed Bordetella pertussis bacteria, precipitated together in alum. IgG1 antibody to CGG and IgG2c antibody to B pertussis were measured in serum of individual mice (dots) by ELISA 14 days after primary immunization, showing normal primary T-dependent antibody formation and polarization. After 37 days, the mice received a booster immunization of CGG-ARS and NP hapten coupled to ficoll, and 7 days later (d44) serum antibodies to CGG, ARS and NP were measured, showing failure to sustain CGG antibody, failure of affinity maturation to produce ARS antibody, but normal T cell independent type 2 antibody response to NP-ficoll.
primurus homozygous mice (pri/pri), and heterozygous (pri/+) or wild-type (+/+) siblings were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS), and heat-killed Bordetella pertussis bacteria, precipitated together in alum. IgG1 antibody to CGG and IgG2c antibody to B pertussis were measured in serum of individual mice (dots) by ELISA 14 days after primary immunization, showing normal primary T-dependent antibody formation and polarization. After 37 days, the mice received a booster immunization of CGG-ARS and NP hapten coupled to ficoll, and 7 days later (d44) serum antibodies to CGG, ARS and NP were measured, showing failure to sustain CGG antibody, failure of affinity maturation to produce ARS antibody, but normal T cell independent type 2 antibody response to NP-ficoll.
Captain morgan homozygous mutant mice (cpm/cpm), primurus homozygous mice (pri/pri), compound heterozygotes between the two strains (cpm/pri), and wild-type control mice (+/+) were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS). Antibodies to CGG and to ARS were measured in serum of individual mice (dots) by ELISA, 14 and 28 days after primary immunization. Antibody titres are shown relative to a reference immune serum pooled from wild-type mice.
Captain morgan homozygous mutant mice (cpm/cpm), primurus homozygous mice (pri/pri), compound heterozygotes between the two strains (cpm/pri), and wild-type control mice (+/+) were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS). Antibodies to CGG and to ARS were measured in serum of individual mice (dots) by ELISA, 14 and 28 days after primary immunization. Antibody titres are shown relative to a reference immune serum pooled from wild-type mice.
Strain identification
How is this strain characterised? Genotyping
PCR protocols Primurus (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • memory
  • antibody
  • immunization
  • ENU
  • Wellcome Trust
  • B cell
  • GTP
Additional Information Discovered by Wellcome Trust Strategic Award. Identified in immunization antibody response screen by Ms Edyta Kucharska and Dr Carola Vinuesa (JCSMR, ANU), mapped and mutation identified by Dr Katrina Randall and Belinda Whittle (JCSMR, ANU) and Dr Teresa Lambe (Oxford), selective defect in antibody maturation established by Dr Katrina Randall (JCSMR, ANU). Project guided by Prof Richard Cornall and Prof Chris Goodnow.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 6 mice)

APB stock genotyped verified by: The depositor
Are live mice available? Unknown
MTA needs to be signed? Yes


     

 


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