Home - Australian Phenomics Facility

Mouse Strain information

APB ID

4113

Added

05/12/2008

Last Edited

10/04/2014

Strain nomenclature:

C57BL/6JAnu-Zbtb7bhpls/AnuApb

Nickname:

Helpless, ENU9B6:035

Strain designation name:

C57BL/6JAnu-Zbtb7bhpls/AnuApb

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: zinc finger and BTB domain containing 7B

Chromosome

Affected Gene Symbol

Zbtb7b

Protein expression of altered gene:

Unknown

Genetic alteration of gene:

ENU-induced mutation.

Location on chromosome (bp):

89181566-89198698 bp, - strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:102755

Synonyms

c-Krox, Th-POK, Thpok, Zfp67

Allele Name

zinc finger and BTB domain containing 7B; helpless

Allele Symbol

Zbtb7bhpls

MGI Allele Accession ID

MGI:3838356

Allele synonyms:

ENU9B6:035

Ensembl Gene ID:

ENSMUSG00000028042

Transcript ID:

ENSMUST00000029677

Transcript name:

Zbtb7b-001

bp change:

T to G at position 480 on cDNA

Exon number:

Exon ID:

ENSMUSE00000175423

cDNA position:

480

Amino acid change:

Leucine to Arginine at position 101

Sequence:
450	ACTTTGTAGGGCCAGAGGCCCTAGGTGCCCTGCTCGAGTTTGCTTACACAGCCACACTGAC	cDNA (reverse strand)
92	D--F--V--G--P--E--A--L--G--A--L--L--E--F--A--Y--T--A--T--L--T	protein

Version:

NCBI m37, Ensembl build 56

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Defective antibody response to immunization: failure to mount a normal primary T cell dependent antibody response. Very few CD4 T cells in peripheral blood or in thymus.

Original Genetic Background

C57BL/6JAnu

Genetic Background Currently Maintained

C57BL/6JAnu

Coat Colour

Black

Strain Images

Animals from pedigree or wild-type C57BL/6 control stocks were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS), and heat-killed Bordetella pertussis bacteria, precipitated together in alum. IgG1 antibody to CGG and IgG2c antibody to B pertussis were measured in serum of individual mice (dots) by ELISA 14 days after primary immunization, showing absence of primary Th2-polarized antibody formation to CGG and Th1-polarized antibody response to B pertussis.

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

9B6 35 (amplifluor)

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Yes	Yes
Are homozygous mice fertile?	Yes	Yes
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Generations Backcrossed	>8
Mating Scheme	Affected Male x C57BL/6JAnu
Homozygous Matings Required?	No
Male Fertility Compromised?	No
Breeding Performance	Unknown
Special Husbandry Requirements	General breeding performance is poor, homozygous females are extremely poor breeders and also hetrozygous females show reduced breeding performance.

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

B cell
T cell, CD4
antibody
ENU
Wellcome Trust

Additional Information

Discovered by Wellcome Trust Strategic Award and by NIH NIAID project. Identified in immunization antibody response screen, and in flow cytometry screen of blood lymphocytes by Edyta Kucharska, Carla Roots and Dr Anselm Enders (JCSMR, ANU). Mutation mapped and identified by Dr Anselm Enders and Belinda Whittle. Project guided by Prof Chris Goodnow.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by:

The APF

Are live mice available?

MTA needs to be signed?

Yes