Mouse Strain information
APB ID 4113
Added 05/12/2008
Last Edited 10/04/2014
Strain name: C57BL/6JAnu-Zbtb7bhpls/AnuApb
Nickname: Helpless, ENU9B6:035
Strain designation name: C57BL/6JAnu-Zbtb7bhpls/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: zinc finger and BTB domain containing 7B  
Chromosome 3
Affected Gene Symbol Zbtb7b
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU-induced mutation.
Location on chromosome (bp): 89181566-89198698 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:102755
Synonyms c-Krox, Th-POK, Thpok, Zfp67
Allele Name zinc finger and BTB domain containing 7B; helpless
Allele Symbol Zbtb7bhpls
MGI Allele Accession ID MGI:3838356
Allele synonyms: ENU9B6:035
Ensembl Gene ID: ENSMUSG00000028042
Transcript ID: ENSMUST00000029677
Transcript name: Zbtb7b-001
bp change: T to G at position 480 on cDNA
Exon number: 2
Exon ID: ENSMUSE00000175423
cDNA position: 480
Amino acid change: Leucine to Arginine at position 101
Sequence:
450ACTTTGTAGGGCCAGAGGCCCTAGGTGCCCTGCTCGAGTTTGCTTACACAGCCACACTGACcDNA (reverse strand)
92D--F--V--G--P--E--A--L--G--A--L--L--E--F--A--Y--T--A--T--L--Tprotein
Version: NCBI m37, Ensembl build 56
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Defective antibody response to immunization: failure to mount a normal primary T cell dependent antibody response. Very few CD4 T cells in peripheral blood or in thymus.
Original Genetic Background C57BL/6JAnu
Genetic Background Currently Maintained C57BL/6JAnu
Coat Colour Black
Strain Images
Animals from pedigree or wild-type C57BL/6 control stocks were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS), and heat-killed Bordetella pertussis bacteria, precipitated together in alum. IgG1 antibody to CGG and IgG2c antibody to B pertussis were measured in serum of individual mice (dots) by ELISA 14 days after primary immunization, showing absence of primary Th2-polarized antibody formation to CGG and Th1-polarized antibody response to B pertussis.
Animals from pedigree or wild-type C57BL/6 control stocks were immunized with chicken gammaglobulin (CGG) coupled to the hapten azobenzene arsonate (ARS), and heat-killed Bordetella pertussis bacteria, precipitated together in alum. IgG1 antibody to CGG and IgG2c antibody to B pertussis were measured in serum of individual mice (dots) by ELISA 14 days after primary immunization, showing absence of primary Th2-polarized antibody formation to CGG and Th1-polarized antibody response to B pertussis.
Strain identification
How is this strain characterised? Genotyping
PCR protocols 9B6 35 (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • B cell
  • T cell, CD4
  • antibody
  • ENU
  • Wellcome Trust
Additional Information Discovered by Wellcome Trust Strategic Award and by NIH NIAID project. Identified in immunization antibody response screen, and in flow cytometry screen of blood lymphocytes by Edyta Kucharska, Carla Roots and Dr Anselm Enders (JCSMR, ANU). Mutation mapped and identified by Dr Anselm Enders and Belinda Whittle. Project guided by Prof Chris Goodnow.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: The APF
Are live mice available? No
MTA needs to be signed? Yes


   

 


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