Mouse Strain information
APB ID 40
Added 06/02/2006
Last Edited 10/05/2014
Strain name: B6;NODAnu-Chkbflp/AnuApb
Nickname: Flipper
Strain designation name: B6;NODAnu-Chkbflp/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: choline kinase beta  
Chromosome 15
Affected Gene Symbol Chkb
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU induced mutation. Results in deletion of first 121 amino acids.
Location on chromosome (bp): 89256798-89260031 bp, - strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1328313
Synonyms Chetk, Chkl, CK/EK-beta
Allele Name flipper
Allele Symbol Chkbflp
MGI Allele Accession ID MGI:3847123
Ensembl Gene ID: ENSMUSG00000022617
Transcript ID: ENSMUST00000023289
Transcript name: Chkb-201
bp change: A to T at position 1 on cDNA
Exon number: 1
Exon ID: ENSMUSE00000322690
cDNA position: 1
Amino acid change: Methionine to Leucine at position 1
Sequence:
1ATGGCGGCAGACGGGACAGGGGTAGTCGGAGcDNA (reverse strand)
1-M--A--A--D--G--T--G--V--V--G--protein
Version: NCBI m37, Ensembl build 55
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygotes develop shortened and lateral rotation (valgus?) of radius and ulna leading to short and splayed forelegs. Female homozygotes can breed. Male homozygotes die suddenly during mating with haemorrhages in seminiferous glands (this is currently being re-investigated). Some homozygotes also develop perineal hernias. Chkbrmd Homozygous null mice display progressive muscular weakness and dystrophy in the hindlimbs but have normal nerve and neuromuscular junction morphology.
Original Genetic Background C57BL/6
Genetic Background Currently Maintained C57BL/6 x NOD
Strain identification
How is this strain characterised? Genotyping
Phenotyping
PCR protocols Flipper (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain Model for understanding mechanisms of muscle degeneration
Applicable Research Areas Developmental biology
Keywords
  • limb
  • rostocaudal muscular dystrophy
  • kinase
  • phosphatidylcholine
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 9 mice)

APB stock genotyped verified by: The APF
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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