Mouse Strain information
APB ID 3940
Added 17/10/2008
Last Edited 28/11/2013
Strain name: C3Arc.MOLD-Oas1bFlv-mr/Arc
Nickname: C3H.DUB/3
Strain designation name: C3Arc.MOLD-Oas1bFlv-mrArc
Genetics
Strain Types Congenic
Genetic Details Spontaneous
Congenic
Mode of Inheritance Dominant
1.  Affected Gene Name: 2'-5' oligoadenylate synthetase 1B  
Chromosome 5
Affected Gene Symbol Oas1b
Protein expression of altered gene: Normal
Genetic alteration of gene: This minor resistance allele differs from other resistance strains by 14 amino acid substitutions.
Location on chromosome (bp): 121262644-121274167 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97430
Synonyms Flavivirus resistance, Flv, L1, Mmu-L1, Oias-2, Oias2, Wnv
Allele Name minor flavivirus resistance
Allele Symbol Oas1bFlv-mr
MGI Allele Accession ID MGI:1857802
Allele synonyms: Flvmr
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Minor resistance to Flavivirus infection. Flvmr Phenotype – Resistance to infections by Flaviviruses. Tlr4 Phenotype – Toll 4 receptor is responsive to LPS injection.
Phenotype Heterozygous State Flvmr Phenotype – dominant phenotype, heterozygotes are resistant to infection. Tlr4 phenotype is dominant and heterozygous mice show responsiveness to LPS.
Original Genetic Background MOLD/RkJ
Genetic Background Currently Maintained C3H/HeJARC
Coat Colour Brown
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols Master Mix B - PCR for congenic region on Chr5 of C3H/HeJARC, C3H.PRI-Flv<r>, C3H.M.domesticus-Flv<r> & C3H.MOLD-Flv<mr> mice
Master Mix A - PCR for congenic region on Chr5 of C3H.M.domesticus-Flv<r> (DUB) and C3H.MOLD-Flv<mr> (MOLD) mice. DUB mice were derived from a wild mouse that was trapped in Dubbo (NSW) and then It was backcrossed to C3H/HeJARC mice for 11 backcross generations, while MOLD mice were created by 10 generations of backcross breeding of an inbred MOLD/Rk mouse to C3H/HeJARC mice.
MOLD
Characteristics Assayed For Flavivirus susceptibility was measured as mortality and brain virus titres following IC inoculation with a Flavivirus (MVE). The phenotyping procedures can be found in Urosevic et al 1999 (see ref 2 and 4 below).
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain This strain is particularly valuable for the virological and immunological characterisation of Flavivirus infection. This strain is resistant to infections and shows moderately reduced mortality and reduced viral titres following inoculation with the virus.
Applicable Research Areas Virology
Keywords
  • Flavivirus resistance
  • Lipopolysaccharide (LPS)
  • Toll like receptor
Additional Information Cryopreserved sperm stored at ARC.
Is the strain available in any form to other researchers Yes
APB stock stored as:

APB stock genotyped verified by: The depositor
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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