Mouse Strain information
APB ID 3686
Added 26/08/2008
Last Edited 10/05/2014
Strain name: C57BL/6JSfdAnu-Cd4thth/AnuApb
Nickname: Thoth ; ENU9B6:064a
Strain designation name: C57BL/6JSfdAnu-Cd4thth/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: CD4 antigen  
Chromosome 6
Affected Gene Symbol Cd4
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU-induced mutation. Sequence: aacttcttttgtatggctt[c/a]gatttgcagGAGGTTCGCC C⇒A, intronic, 10bp upstream of intron 4-exon 4 boundary
Location on chromosome (bp): 124814710-124838239 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:88335
Synonyms L3T4, Ly-4
Allele Name CD4 antigen; thoth
Allele Symbol Cd4thth
MGI Allele Accession ID MGI:4819162
Ensembl Gene ID: ENSMUSG00000023274
Transcript ID: ENSMUST00000024044
Transcript name: Cd4-001
bp change: C to A
Intron number: 3
Genomic position: 124823158
Sequence:
124823188cagggcacctcaacttcttttgtatggcttCgatttgcagGAGGTTCGCCTTCGCAGTTTGAgenomic (reverse strand)
    Exons
Version: NCBI m37, Ensembl build 58
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State No CD4+ T cells in thymus or periphery
Original Genetic Background C57BL/6JAnu
Genetic Background Currently Maintained C57BL/6JAnu
Strain Images
FACS plot from affected (#4) animal compared to unaffected siblings
FACS plot from affected (#4) animal compared to unaffected siblings
Strain identification
How is this strain characterised? Genotyping
PCR protocols 9B6 64 (amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • T cell
  • CD4
  • ENU
  • NIH
  • Wellcome Trust
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? No


   

 


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