Mouse Strain information
APB ID 341
Added 22/09/2006
Last Edited 11/05/2014
Strain name: B6;129-Epha1tm1Sld/Apb
Nickname: EphA1-/- hPLAP
Strain designation name: B6;129-Epha1tm1Sld/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Targeted reporter
ES Cell-Line Used Emery
Mode of Inheritance Recessive
1.  Affected Gene Name: Eph receptor A1  
Chromosome 6
Affected Gene Symbol Epha1
Protein expression of altered gene: Nil
Genetic alteration of gene: A cassette containing an IRES-ALPP reporter gene and floxed neo gene was inserted into exon 2. Germ line, cre mediated recombination was used to remove the neo cassette. The absence of transcript expression was confirmed by QPCR of liver extracts.
Location on chromosome (bp): 42308486-42323267 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:107381
Synonyms 5730453L17Rik, Eph, Esk
Allele Name targeted mutation 1, Shannon L Duffy
Allele Symbol Epha1tm1Sld
MGI Allele Accession ID MGI:3822370
1.   Transgene: internal ribosome entry site - human placental alkaline phosphatase  
Mutant Construction Technique CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State Vaginal inflammation: mice exhibit inflammation of the vaginal mucosa secondary to hydrometrocolpos. Endometrium inflammation: mice exhibit endometrium inflammation secondary to hydrometrocolpos. Dilated uterine horn: mice exhibit dilated, attenuated uterine horns secondary to hydrometrocolpos Enlarged uterus: mice exhibit an enlarged uterus due to an accumulation of clear uterine fluid (hydrometrocolpos). Increased uterus weight: Vagina atresia: 18% of mice exhibit a failure of vaginal opening despite normal estrogen receptor expression, beta-estradiol treatment fails to induce precocious vaginal opening in P12 mice unlike in wild-type mice and vaginal opening can not be induced by likewise treating affected adult mice. Hydrometrocolpos: mice exhibit inflammation of the vaginal mucosa and endometrium with dilated, attenuated uterine horns secondary to hydrometrocolpos Female infertility: female mice affected by hydrometrocolpos are sterile Kinked tail: up to 80% of mice exhibit a kinky tail due to incorrectly positioned caudal vertebrae Distended abdomen: in some mice due to an enlarged uterus Abnormal response/metabolism to endogenous compounds: treatment with beta-estradiol fails to induce apoptosis in the lower genital tract and precocious vaginal opening unlike in wild-type mice.
Phenotype Heterozygous State Normal
Original Genetic Background 129/SvJ x C57BL/6
Genetic Background Currently Maintained C57BL/6
Coat Colour Black / Brown
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Research value of this Strain Investigations into the expression and function of EphA1 protein
Applicable Research Areas Cell biology
Developmental biology
Haematology
Reproduction
Research tools
Apoptosis
Cancer
Keywords
  • Eph
  • Receptor Tyrosine Kinase
  • ephrin
  • epithelium
  • uterus
  • tail
  • hydrometrocolpos
Additional Information Two lines were generated from 2 separate ES cell clones. Both have been frozen down.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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