Mouse Strain information
APB ID 3395
Added 11/07/2008
Last Edited 11/05/2014
Strain name: C3H/HeJArcApb
Nickname: C3H/HeJARC
Strain designation name: C3H/HeJArcApb
Genetics
Strain Types Congenic
Mode of Inheritance Recessive
1.  Affected Gene Name: 2'-5' oligoadenylate synthetase 1B  
Chromosome 5
Affected Gene Symbol Oas1b
Protein expression of altered gene: Normal
Genetic alteration of gene: This allele confers susceptibility to flavivirus infection. Susceptible mouse strains produce a protein lacking 30% of the C-terminal region due a nucleotide substitution. The C820T transition results in a premature stop codon.
Location on chromosome (bp): 121262644-121274167 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97430
Synonyms Flavivirus resistance, Flv, L1, Mmu-L1, Oias-2, Oias2, Wnv
Allele Name 2'-5' oligoadenylate synthetase 1B; flavivirus susceptibility
Allele Symbol Oas1bFlv-s
MGI Allele Accession ID MGI:2181524
Allele synonyms: Flv-s
2.  Affected Gene Name: dopamine-induced hypothermia  
Chromosome 5
Affected Gene Symbol Diht
Protein expression of altered gene: Unknown
Genetic alteration of gene: Resistance to apomorphine-induced hypothermia compared to C3.PRI-Oas1bFlv-r.
Location on chromosome (bp): 115413178-115413490 bp, + strand
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:3033372
Allele Name C3H/HeJArc
Allele Symbol DihtC3H/HeJArc
MGI Allele Accession ID MGI:3586687
3.  Affected Gene Name: toll-like receptor 4  
Chromosome 4
Affected Gene Symbol Tlr4
Genetic alteration of gene: This revertant to wild-type of the Tlr4Lps-d, defective lipopolysaccharide response, allele was identified in a screen for LPS responsiveness of several C3H/He sublines maintained at Australian facilities. Sequence analysis of Tlr4 in the vicinity of the original mutation, which replaced C with A at nucleotide position 2342 and changed a triplet (CCT) encoding proline with a codon (CAT) for histidine in the third exon, demonstrated that C3H/HeJArc and its derivative congenic strains bear a Tlr4 allele identical in sequence to wild-type. As Tlr4Lps-d occurred in C3H/HeJ between 1958 and 1965 (J:5919) and C3H/HeJArc has been maintained at the Animal Resources Centre in Murdoch, WA, Australia since no earlier than 1971 (J:169915), the apparent wild-type allele in this strain is presumed to have arisen by reverse mutation.
Location on chromosome (bp): 66488618-66591318 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:96824
Synonyms lipopolysaccharide response, Lps, Rasl2-8
Allele Name toll-like receptor 4; wild type revertant
Allele Symbol Tlr4Lps-d+
MGI Allele Accession ID MGI:4943781
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Flv phenotype:- Susceptible to infections by Flaviviruses. Tlr4 Phenotype:- responsiveness of Toll 4 Receptor to LPS infection. Diht Phenotype:- a decreased hypothermia response as measured by drop in core temperature following the peripheral administration of a dopamine agonist
Phenotype Heterozygous State Flvs Phenotype:- recessive phenotype, hets are resistant to infection by Flaviviruses. Tlr4 phenotype:- is dominant and heterozygous mice show responsiveness to LPS. Dihtlow phenotype is recessive and dopamine-induced hypothermia in heterozygotes show the high responder type.
Original Genetic Background C3H/HeJ
Genetic Background Currently Maintained C3H/HeJArc
Coat Colour Brown
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols Chr5 of C3H/HeJarc, C3H.PRI-Flvr & C3h.PRI-Dihthigh Mice
Characteristics Assayed For Hypothermia phenotype was performed by taking rectal temperatures following peripheral administration of a dopamine agonist. Protocol can be found in Stoddart et al 2004. Flavivirus susceptibility was measured as mortality and brain virus titres following IC inoculation with a Flavivirus (MVE). See Urosevic et al 1997. Assessment of LPS responsiveness described in Silvia and Urosvic 1999.
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain This strain forms part of a comparative congenic model used to investigate flavivirus susceptibility/resistance and dopamine related phenotypes. This strain shows susceptibility to flavivirus infection and is the genetic background control strain for congenic C3H strains resistant to flaviviruses. The hypothermia model has been developed using dopamine-induced hypothermia as the phenotypic marker and C3H/HeJARC responses show reduced dopamine-induced hyopthermia compared to mice possessing the Dihthigh allele.
Applicable Research Areas Virology
Neurobiology
Keywords
  • dopamine
  • flavivirus resistance
  • apomorphine
  • hypothermia
  • lipopolysaccharide (LPS)
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


Build: 2017.09.11 Non-profit supported by the Australian government  |  © Copyright 2017 Australian Phenomics Facility Last updated: 22/09/2017