Mouse Strain information
APB ID 3392
Added 11/07/2008
Last Edited 11/05/2014
Strain name: C3.PRI-Oas1bFlv-r/ArcApb
Nickname: C3H.PRI-Flvr; C3H/Rv
Strain designation name: C3.PRI-Oas1bFlv-r/ArcApb
Genetics
Strain Types Congenic
Genetic Details Spontaneous
Mode of Inheritance Dominant
1.  Affected Gene Name: 2'-5' oligoadenylate synthetase 1B  
Chromosome 5
Affected Gene Symbol Oas1b
Protein expression of altered gene: Normal
Genetic alteration of gene: This allele confers resistance to flavivirus infection.
Location on chromosome (bp): 121262644-121274167 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97430
Synonyms Flavivirus resistance, Flv, L1, Mmu-L1, Oias-2, Oias2, Wnv
Allele Name flavivirus resistance
Allele Symbol Oas1bFlv-r
MGI Allele Accession ID MGI:1857800
Allele synonyms: Flvr
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Decreased susceptibility to viral infection: viral replication was inhibited in mice from resistant strains upon innoculation with the following viruses: Yellow Fever, Dengue Fever, West Nile Fever, Japanese Encephalitis, St. Louis Encephalitis, Russian Spring Summer Encephalities. Flvr Phenotpye:- resistance to infections by Flaviviruses. Tlr4 Phenotpye:- Toll 4 receptor is responsive to LPS infection. Dihthigh Phenotpye:- an increased hypothermia response as measured by a drop in core temperature following peripheral administration of a dopamine agonist.
Phenotype Heterozygous State Flvr Phenotpye:- dominant phenotype, heterozygotes are resistant to infection. Tlr4 Phenotpye:- dominant phenotype, heterzygotes show responsiveness to LPS. Dihthigh Phenotpye:- dominant and dopamine-induced hypothermia in heterozygotes show the high responder phenotype.
Original Genetic Background C3H/HeJ
Genetic Background Currently Maintained C3.PRI.Oas1bFlv-r
Coat Colour Brown
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols Chr5 of C3H/HeJarc, C3H.PRI-Flvr & C3h.PRI-Dihthigh Mice
Characteristics Assayed For Hypothermia phenotype was performed by taking rectal temperatures following peripheral administration of a dopamine agonist. Protocol can be found in Stoddart et al 2004. Flavivirus susceptibility was measured as mortality and brain virus titres following IC inoculation with a Flavivirus (MVE). See Urosevic et al 1997. Assessment of LPS responsiveness described in Silvia and Urosvic 1999.
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain This strain is particularly valuable for the virological and immunological characterisation of Flavivirus infection. Tis strain is resistant to infections and shows significantly reduced mortality and reduced viral titres following inoculation with the virus. This C3H.PRI-Flvr strain also forms part of a comparative congenic model used to investigate dopamine related phenotypes. The model has been developed using dopamine-induced hypothermia as the phenotypic marker. This strain also exhibits hyper-dopaminergic responses in other phenotypes, such as the startle response and prepulse inhibition of the startle response (models for sensorimotor gating).
Applicable Research Areas Virology
Neurobiology
Keywords
  • dopamine
  • flavivirus resistance
  • apomorphine
  • hypothermia
  • lipopolysaccharide (LPS)
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


   

 


Build: 2017.09.11 Non-profit supported by the Australian government  |  © Copyright 2017 Australian Phenomics Facility Last updated: 22/09/2017