Mouse Strain information
APB ID 3340
Added 20/06/2008
Last Edited 11/05/2014
Strain name: B6.CBACa-Tg(CD2-Il5)5C2Ldt/Apb [cc]
Nickname: C57BL/6 Tg(IL5)C2
Strain designation name: B6.CBACa-Tg(CD2-Il5)5C2Ldt/Apb [cc]
Genetics
Strain Types Mutant strain
Congenic
Genetic Details Transgenic
Mode of Inheritance Dominant
1.   Transgene: transgene insertion 5C2, Lindsay A Dent  
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Constitutive expression of the IL5 gene is sufficient to induce lifelong high-level eosinophilia. Northern blot analysis identified 11,5 mRNA in the thymus, Peyer's patches, and subcutaneous (pooled inguinal and axillary) lymph nodes. No IL5 mRNA was detected in liver, heart, brain, or skeletal muscle tissues from transgenics, nor in any tissues from normal littermates. Do not maintain as homozygotes. While this is apparently possible, survival of homozygotes likely to be lower.
Phenotype Heterozygous State C57BL/6 IL-5 Tg2 mice are resistant to methylcholanthrene-induced carcinogenesis - see Simson, L, et al., 2007, Regulation of carcinogenesis by IL-5 and CCL11: a potential role for eosinophils in tumor immune surveillance. J. Immunol., 178:4222-4229.
Original Genetic Background CBA/Ca
Genetic Background Currently Maintained C57BL/6
Coat Colour Black
Eye Colour Black
Strain identification
How is this strain characterised? Phenotyping
Characteristics Assayed For Routinely phenotype IL-5 Tg mice by counting cells from tail blood smears, with eosinophils being the predominant cell type present if the mouse is IL-5 Tg. All mice that will be sent for preservation have been identified as IL-5 Tg through a blood smear taken at weaning and then again a few weeks later to confirm the IL-5 Tg status. We do not usually use PCR.
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Yes
Research value of this Strain Hypereosinophilia, atopy, allergy, asthma, parasite infection, cancer
Applicable Research Areas Models for human disease
Cell biology
Developmental biology
Haematology
Reproduction
Dermatology
Apoptosis
Cancer
Immunology and inflammation
Keywords
  • eosinophil
  • immune surveillance
  • esistance to helminthic parasites
  • asthma
  • allergy
Additional Information IL-5 Tg mice have enhanced resistance to some parasitic helminths (Nippostrongylus brasiliensis, Strongyloides ratti), but not to others (Schistosoma mansoni, Trichinella spiralis, Toxocara canis). Expression of the IL-5 Tg on CBA/Ca, C57BL/6 and BALB/c genetic backgrounds enhances the immunopathology of aeroallergen-induced lung inflammation, but the CBA/Ca mice are relatively resistant to development of several aspects of the disease. Mammary tissue development is temporarily retarded in IL-5 Tg mice.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


   

 


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