Mouse Strain information
APB ID 3324
Added 15/06/2008
Last Edited 11/05/2014
Strain name: B6;129S1-Tgm2tm1Rmgr/Apb
Nickname: TGM2 floxed
Strain designation name: B6;129S1-Tgm2tm1Rmgr/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted floxed
ES Cell-Line Used W9.5/W95
Mode of Inheritance Recessive
1.  Affected Gene Name: transglutaminase 2, C polypeptide  
Chromosome 2
Affected Gene Symbol Tgm2
Protein expression of altered gene: Normal
Genetic alteration of gene: LoxP sites that flank exons 6-8 were inserted by homologous recombination.
Location on chromosome (bp): 157942138-157972172 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:98731
Synonyms G[a]h, protein-glutamine gamma-glutamyltransferase, TG C, TG2, tissue transglutaminase, tTG, tTGas
Allele Name targeted mutation 1, Robert M Graham
Allele Symbol Tgm2tm1Rmgr
MGI Allele Accession ID MGI:3525256
Allele synonyms: Tgm2t
Mutant Construction Technique CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State Normal
Phenotype Heterozygous State Normal
Original Genetic Background 129S1/SvImJ x C57BL/6
Genetic Background Currently Maintained 129S1/Sv x C57BL/6
Coat Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols TGM2 flox
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? Yes
Does it Model a Human Condition? Unknown
Research value of this Strain Many important biological processes, such as blood coagulation, are dependent on rapid cross-linking or "spotwelding". These reactions, which are catalysed by transglutaminases, endow the resulting structure with extra rigidity and resistance against proteolytic degradation. Having knowledge of these enzymes is essential for understanding diverse hereditary diseases of the blood and skin and various autoimmune, inflammatory and degenerative conditions. One such enzyme, tranglutaminase type 2 (TGM2) has been implicated in wound healing and in the pathogenesis of inflammatory disorders such as Crohn's disease, ulcerative colitis and coeliac disease as well as a number of important neurodegenerative diseases, such as Huntington's, Alzheimer's and progressive suprabulbar palsy. TG2 is unique among the transglutaminases because it is the only member shown to have functions other than cross-linking. The various functions of TG2 must be tightly coordinated. To better understand the function of TG2, we utilised homologous recombination to develop a floxed mouse line in which the TGM2 gene can be inactivated by Cre-mediated excision, either in the whole organism or in tissue-specific manner.
Applicable Research Areas Diabetes
Apoptosis
Neurobiology
Cancer
Cardiovascular
Immunology and inflammation
Keywords
  • Transgluatminase
  • Gh
  • G-protein
  • cross-linking
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


   

 


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