Mouse Strain information
APB ID 30
Added 06/02/2006
Last Edited 11/05/2014
Strain name: C57BL/6JSfdAnu-Trp53Bbl/AnuApb
Nickname: B-blast
Strain designation name: C57BL/6JSfdAnu-Trp53Bbl/AnuApb
Genetics
Strain Types Mutant strain
Coisogenic
Genetic Details Chemically induced : ENU
Mode of Inheritance Semi-dominant
1.  Affected Gene Name: transformation related protein 53  
Chromosome 11
Affected Gene Symbol Trp53
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU induced mutation.
Location on chromosome (bp): 69393861-69405375 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:98834
Synonyms p53
Allele Name b-blast
Allele Symbol Trp53Bbl
MGI Allele Accession ID MGI:3611496
Ensembl Gene ID: ENSMUSG00000059552
Transcript ID: ENSMUST00000108658
Transcript name: Trp53-201
bp change: T to A at position 1111 on cDNA
cDNA position: 1111
Amino acid change: Isoleucine to Asparagine at position 195
Version: NCBI m37, Ensembl build 55
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygotes develop B cell leukaemia/lymphoma or primitive haemopoietic leukaemia at 4-6 mths.
Phenotype Heterozygous State Heterozygotes develop solid tumours osteosarcoma, lymphoma, haemangiosarcoma, fibrosarcoma, thymic lymphoma, multinodal lymphoma, brain tumour and teratocarcinoma at 8-15mths.
Original Genetic Background C57BL/6JSfdAnu
Genetic Background Currently Maintained C57BL/6JSfdAnu
Coat Colour Black
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols B_blast (amplifluor)
B-Blast (Bbl)-Mutant band
B-Blast (Bbl) wt band
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
General information
Associated IP rights? No
Does it Model a Human Condition? Yes
Applicable Research Areas Cancer
Keywords
  • p53
  • apoptosis
  • tumour
  • cell cycle
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 13 mice)

APB stock genotyped verified by: The APF
Are live mice available? No
MTA needs to be signed? Yes


   

 


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