Mouse Strain information
APB ID 267
Added 30/04/2006
Last Edited 11/05/2014
Strain name: C57BL/6-Gzmatm1Simn/AnuApb
Nickname: B6.gA-/-
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: granzyme A  
Chromosome 13
Affected Gene Symbol Gzma
Protein expression of altered gene: Nil
Genetic alteration of gene: Resistance cassette into exon 4 by homologous recombination. Western blot analysis confirmed the absence of protein product and cytochemical analysis demonstrated the absence of enzyme activity in cytotoxic T cells in homozygous mutant animals.
Location on chromosome (bp): 114214703-114221857 bp, - strand
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:109266
Synonyms BLT esterase, Ctla-3, Ctla3, H factor, Hanukah factor, Hf, SE1, serine esterase 1, TSP-1, TSP1
Allele Name targeted mutation 1, Markus M Simon
Allele Symbol Gzmatm1Simn
MGI Allele Accession ID MGI:2449926
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Tumorigenesis * homozygotes control growth of syngeneic tumors (s.c. inoculation of H-2b MC57G fibrosarcoma) as efficiently as wild-type mice Hematopoietic system phenotype * homozygotes are viable, fertile, and display normal hematopoiesis relative to wild-type mice * in mutant spleens, the numbers of CD4+ and CD8+ T cells, macrophages, NK cells and B cells fall within normal ranges * the frequencies of CD4+ and CD8+ T cells responding to H-2 alloantigens in primary mixed lymphocyte cultures (MLC) with proliferation are also within normal ranges Immune system phenotype * in vitro- and ex vivo-derived cytotoxic T cells and NK cells from mutant mice induce specific target cell membrane disruption, DNA fragmentation, and apoptosis as efficiently as wild-type cells * homozygotes control infections with lymphocytic choriomeningitis (LCM) virus and L. monocytogenes as efficiently as wild-type mice * homozygotes develop a normal contact hypersensitivity (CHS) response (based on increase in ear thickness) to trinitrophenyl (TNP) relative to wild-type mice
Original Genetic Background C57BL/6
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
PCR protocols FasAB_GranzymeA(ASD)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Haematology
Cell biology
Immunology and inflammation
Keywords
  • Cytotoxic T Cell
  • Natural Killer Calls
  • Serine Proteinase
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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