Mouse Strain information
APB ID 2444
Added 14/01/2008
Last Edited 10/05/2014
Strain name: C57BL/6-Ptprcltng/AnuApb
Nickname: Lightning ; ENU8B6:045
Strain designation name: C57BL/6-Ptprcltng/Apb
Genetics
Strain Types Mutant strain
Coisogenic
Genetic Details Chemically induced : ENU
Mode of Inheritance Recessive
1.  Affected Gene Name: protein tyrosine phosphatase, receptor type, C  
Chromosome 1
Affected Gene Symbol Ptprc
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU-induced mutation. ENU induced at T to C transition that results in the amino acid substitution of serine for phenylalanine at position 528 (F528S). Flow cytometry confirmed that protein half-life is reduced.
Location on chromosome (bp): 139959992-140071843 bp, - strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97810
Synonyms B220, CD45, Cd45, Ly-5, Lyt-4, T200
Allele Name protein tyrosine phosphatase, receptor type, C; lightening
Allele Symbol Ptprcltng
MGI Allele Accession ID MGI:4441612
Allele synonyms: PtprcL
Ensembl Gene ID: ENSMUSG00000026395
Transcript ID: ENSMUST00000027645
Transcript name: Ptprc-201
bp change: T to C at position 1681 on cDNA
Exon number: 14
Exon ID: ENSMUSE00000595907
cDNA position: 1681
Amino acid change: Phenylalanine to Serine at position 528
Sequence:
1651TTACAAAATACAACAAGACAAACTGTCAGTTTTATGTAGATAATCTCTACTATTCAACTGAcDNA (reverse strand)
518V--T--K--Y--N--K--T--N--C--Q--F--Y--V--D--N--L--Y--Y--S--T--Dprotein
Version: NCBI m37, Ensembl build 57
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Homozygous null mutants have defective T-cell maturation. Decreased CD4-positive T cell number: SP4 thymocytes are reduced compared to in wild-type mice. Abnormal T cell differentiation: * mice exhibit enhanced negative selection compared with wild-type mice. * positive selection of T cell is rescued and single positive thymic subsets compared to in Ptprctm1Mak homozygotes. Decreased double-positive T cell number. Thymus hypoplasia.
Original Genetic Background C57BL/6
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Cell biology
Immunology and inflammation
Keywords
  • T cell
  • Ly5
  • thymocyte, lymphocyte
  • B cell
  • ENU
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 3 mice)

APB stock genotyped verified by: The depositor
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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