Mouse Strain information
APB ID 2433
Added 13/01/2008
Last Edited 11/05/2014
Strain name: B6;129P2-Cd22tm1Eac Tg(IghelMD4)4Ccg/AnuApb
Nickname: CD22-/-:Hamlet
Strain designation name: B6;129P2-Cd22tm1Eac Tg(IghelMD4)4Ccg/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Transgenic
Mode of Inheritance Recessive
1.  Affected Gene Name: CD22 antigen  
Chromosome 7
Affected Gene Symbol Cd22
Protein expression of altered gene: Nil
Genetic alteration of gene: Portions of exons 3 and 4 were replaced with a neomycin selection cassette, which introduced stop codons into all three reading frames. The deleted region encoded the translational start site, leader sequence, and the ligand-binding immunoglobulin domain 1. The splice sites on the 5' and 3' terminals of exons 3 and 4, respectively, were left intact.
Location on chromosome (bp): 31650848-31665361 bp, - strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:88322
Synonyms Lyb-8, Lyb8
Allele Name CD22 antigen; targeted mutation 1, Edward A Clark
Allele Symbol Cd22tm1Eac
MGI Allele Accession ID MGI:2180680
1.   Transgene: transgene insertion 4, Christopher C Goodnow  
Mutant Construction Technique None
Phenotype
Phenotype Heterozygous State Abnormal mature B cell morphology: * expression of CD22 on the surface of cells is half that of controls * surface expression of IgM is about 70-80% of controls
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Applicable Research Areas Cell biology
Immunology and inflammation
Keywords
  • B cell
  • T cell
  • lymphocyte
  • signalling
  • B cell antigen receptor
  • autoimmunity
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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