Mouse Strain information
APB ID 2432
Added 13/01/2008
Last Edited 11/05/2014
Strain name: B6;129P2-Icosltm1Kplm/AnuApb
Nickname: B7h knockout (ICOS ligand)
Strain designation name: B6;129P2-Icosltm1Kplm/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: icos ligand  
Chromosome 10
Affected Gene Symbol Icosl
Protein expression of altered gene: Nil
Genetic alteration of gene: Sequence encompassing a portion of the promoter and exons encoding the initiator methionine, signal peptide, and IgV-like domain were replaced with a neomycin resistance gene inserted by homologous recombination. Neither full length nor truncated transcript was detected by Northern blot or RT-PCR analyses of message isolated from homozygous mutant spleen tissue.
Location on chromosome (bp): 77532113-77542270 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1354701
Synonyms B7-H2, B7h, B7RP-1, GL50, GL50-B, ICOS-L, LICOS
Allele Name targeted mutation 1, Kong-Peng Lam
Allele Symbol Icosltm1Kplm
MGI Allele Accession ID MGI:2673557
Allele synonyms: B7-H2-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Mice homozygous for disruptions in this gene exhibit defects in the humoral immune response associated with an impaired interactions between T and B cells. impaired germinal center formation. impaired interaction between T and B cells while normal repsonses are mounted against both type I and type II Tcell-independent antigens, the response to T-cell-dependent antigen was attenuated
Original Genetic Background 129
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
PCR protocols B7H KO
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Applicable Research Areas Immunology and inflammation
Keywords
  • B cell
  • T cell
  • lymphocyte
  • Germinal centre
  • costimulation
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 8 mice)

APB stock genotyped verified by: The depositor
Are live mice available? No
MTA needs to be signed? Yes


   

 


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