Mouse Strain information
APB ID 2418
Added 09/01/2008
Last Edited 11/05/2014
Strain name: C57BL/6JAnu-Gnaztm1Iah/AnuApb
Nickname: Gz alpha knockout
Strain designation name: C57BL/6JAnu-Gnaztm1Iah/AnuApb
Genetics
Strain Types Mutant strain
Coisogenic
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: guanine nucleotide binding protein, alpha z subunit  
Chromosome 10
Affected Gene Symbol Gnaz
Protein expression of altered gene: Nil
Genetic alteration of gene: The alpha subunit was ablated by the insertion of a PGK-neo cassette 160 bp downstream of the translation start site. Aplha subunit protein was absent in Western blots of brains and other tissues of mutant mice.
Location on chromosome (bp): 74429922-74479652 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:95780
Synonyms Gz
Allele Name guanine nucleotide binding protein, alpha z subunit; targeted mutation 1, I A Hendry
Allele Symbol Gnaztm1Iah
MGI Allele Accession ID MGI:3575007
Allele synonyms: Gzalpha-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Decreased sensitivity to xenobiotic induced morbidity/mortality: * morphine treated homozygotes are able to tolerate higher morphine doses compared to wild-type mice (LD50 of 800 mg/kg compared to 700 mg/kg for wild-type). * however no significant difference in the serum levels of morphine or its metabolites is seen. Decreased chemically-elicited antinociception: * in previously unexposed female homozygotes reduced analgesia at low morphine doses is seen, a similar but not significant effect was seen in males. * with repeated morphine doses mutants develop tolerance faster than wild-type mice. * acute morphine administration induces the same degree of antinociception; however homozygotes develop tolerance to morphine more rapidly compared to wild-type mice in the hot and cold plate tests. * spinal mechanisms of morphine tolerance are normal Reduced fertility: litters from homozygous parents on the C57BL/6 background had increased mortality that was not seen in litters from heterozygous or mixed strain parents. Decreased body weight: * body weight is normal at birth but homozygotes gain less weight and males and females are 22% and 27% smaller than their wild-type littermates at 2 - 3 weeks of age. * After 3 weeks of age homozygotes gain weight more rapidly and the body weight difference is gone by 2 - 3 months of age.
Phenotype Heterozygous State Abnormal chemically-elicited antinociception: with repeated morphine doses heterozygotes develop tolerance faster than wild-type mice but not as quickly as homozygous mutants. Decreased sensitivity to xenobiotic induced morbidity/mortality: morphine treated homozygotes are able to tolerate higher morphine doses compared to wild-type mice (LD50 of 750 mg/kg compared to 700 mg/kg for wild-type).
Original Genetic Background C57BL/6JAnu
Genetic Background Currently Maintained C57BL/6jAnu
Coat Colour Black
Eye Colour Brown
Strain identification
How is this strain characterised? Genotyping
PCR protocols GαZ KO
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Research value of this Strain Increased tolerance to opiates
Applicable Research Areas Developmental biology
Keywords
  • G-protein
  • Tolerance
  • Feeding Behaviour
  • xenobiotic
  • morphine
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 11 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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