Mouse Strain information
APB ID 2397
Added 20/12/2007
Last Edited 11/05/2014
Strain name: B6;129X1-Zap70tm1Weis/AnuApb
Nickname: Zap70 knockout
Strain designation name: B6;129X1-Zap70tm1Weis/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: zeta-chain (TCR) associated protein kinase  
Chromosome 1
Affected Gene Symbol Zap70
Protein expression of altered gene: Nil
Genetic alteration of gene: A neomycin resistance cassette replaced a genomic fragment containing sequences corresponding to amino acids 221 to 520, which encode the C-terminal SH2 domain. Western blot analysis on thymocytes derived from homozygous mice confirmed that no detectable protein is expressed from this allele.
Location on chromosome (bp): 36818706-36839661 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:99613
Synonyms Srk, TZK, ZAP-70
Allele Name targeted mutation 1, Arthur Weiss
Allele Symbol Zap70tm1Weis
MGI Allele Accession ID MGI:2176252
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Abnormal T cell differentiation: * the number of dendritic epidermal T cells is reduced and those cells present lack the characteristic dendritic extensions. * few intestinal intraepithelial lymphocytes express gamma delta or alpha beta T cell receptors. Arrested T cell differentiation: arrest at the double positive stage Abnormal splenic cell ratio: spleens are composed of mostly B cells. Abnormal lymph node cell ratio: * lymph nodes contain mostly B cells and a significant percentage of CD3+ (but CD4- and CD8-) cells which are primarily gamma delta T cells. * the percentage of gamma delta T cells is increased but the absolute number of these cell is similar to wild-type.
Original Genetic Background 129X1/SvJ
Genetic Background Currently Maintained C57BL/6 x 129X1
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Applicable Research Areas Internal/organ
Immunology and inflammation
Keywords
  • T cell
  • lymphocyte
  • B cell
  • lymph node
  • spleen
  • differentiation
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 2 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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