Mouse Strain information
APB ID 2369
Added 14/12/2007
Last Edited 09/05/2014
Strain name: C57BL/6JSfdAnu-Prkcbtilc/AnuApb
Nickname: Tilcara ; ENU6WT:121
Strain designation name: C57BL/6JSfdAnu-Prkcbtilc/AnuApb
Genetics
Strain Types Mutant strain
Genetic Details Chemically induced : ENU
Mode of Inheritance Semi-dominant
1.  Affected Gene Name: protein kinase C, beta 1  
Chromosome 7
Affected Gene Symbol Prkcb1
Protein expression of altered gene: Unknown
Genetic alteration of gene: ENU-induced mutation. T ➞C in Exon 15 resulting in S 552➡P
Location on chromosome (bp): 129432265-129777916 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:97596
Synonyms PKC-Beta, Pkcb, Prkcb2
Allele Name tilcara
Allele Symbol Prkcbtilc
MGI Allele Accession ID MGI:4819152
Allele synonyms: ENU6WT:121
Ensembl Gene ID: ENSMUSG00000052889
Transcript ID: ENSMUST00000064989
Transcript name: Prkcb-001
bp change: T to C at position 1874 on cDNA
Exon number: 15
Exon ID: ENSMUSE00000439236
cDNA position: 1874
Amino acid change: Serine to Proline at position 552
Sequence:
1844GAAGGGGAGGATGAGGATGAACTCTTCCAGTCAATCATGGAACACAATGTGGCGTATCCCAcDNA (forward strand)
542-E--G--E--D--E--D--E--L--F--Q--S--I--M--E--H--N--V--A--Y--P--protein
Version: NCBI m37, Ensembl build 56
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Defective T-cell independent type 2 antibody response to NP-Ficoll.
Original Genetic Background C57BL/6JSfdAnu
Genetic Background Currently Maintained C57BL/6JSfdAnu
Coat Colour Black
Strain identification
How is this strain characterised? Genotyping
PCR protocols Tilcara_(2007) New Hot Start
Tilcara (Amplifluor)
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Immunology and inflammation
Keywords
  • B cell
  • protein kinase
  • signal transduction
  • ENU
  • Wellcome Trust
Additional Information Discovered by Wellcome Trust Immunization Screen. Identified in immunization antibody response screen by Ms Edyta Kucharska and Dr Anselm Enders (JCSMR, ANU). Mutation mapped and identified by Charis Teh, Belinda Whittle and Dr Anselm Enders. Project guided by Prof Chris Goodnow.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 6 mice)

APB stock genotyped verified by: Unverified
Are live mice available? No
MTA needs to be signed? Yes


   

 


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