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Mouse Strain information

APB ID

2367

Added

14/12/2007

Last Edited

10/05/2014

Strain nomenclature:

B6;CB-Prkdchanky/AnuApb

Nickname:

Mr Hankey ; ENU8CAT:005

Strain designation name:

B6;CB-Prkdchanky/AnuApb

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: protein kinase, DNA activated, catalytic polypeptide

Chromosome

Affected Gene Symbol

Prkdc

Protein expression of altered gene:

Unknown

Genetic alteration of gene:

ENU-induced mutation

Location on chromosome (bp):

15637959-15842332 bp, + strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:104779

Synonyms

DNA-PK, DNA-PKcs, DNAPDcs, slip, XRCC7

Allele Name

hankey

Allele Symbol

Prkdchanky

MGI Allele Accession ID

MGI:4820716

Allele synonyms:

mr hankey

Ensembl Gene ID:

ENSMUSG00000022672

Transcript ID:

ENSMUST00000023352

Transcript name:

Prkdc-201

bp change:

T to A at position 1657 on cDNA

Exon number:

Exon ID:

ENSMUSE00000282431

cDNA position:

1657

Amino acid change:

Leucine to STOP at position 545

Sequence:
1627	TAGGCTGTGACCAGATGGAGGATTTTATTTTAGGAGATGAAACATTTCTCTTTGTGAACTC	cDNA (forward strand)
535	L--G--C--D--Q--M--E--D--F--I--L--G--D--E--T--F--L--F--V--N--S	protein

Version:

NCBI m37, Ensembl build 57

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Diarrhoea. Lymphopenia

Original Genetic Background

C57BL/6 x CBA

Genetic Background Currently Maintained

C57BL/6JAnu x CBA/CaJ

Strain identification

How is this strain characterised?

Genotyping
Phenotyping

PCR protocols

Mr Hankey (amplifluor)

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Gastrointestinal
Developmental biology

Keywords

gut
diarrhoea
intestine
ENU

Is the strain available in any form to other researchers

APB stock stored as:

Cryopreserved sperm (from 4 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

Unknown

MTA needs to be signed?

Yes