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Mouse Strain information
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| APB ID | 2360 | |||
| Added | 13/12/2007 | |||
| Last Edited | 17/04/2014 | |||
| Strain name: | C57BL/10-Tg(Cd2-TcraF5,CD2-TcrbF5)1Kio/AnuApb | |||
| Nickname: | TCRF5 | |||
| Strain designation name: | C57BL/10-Tg(Cd2-TcraF5,CD2-TcrbF5)1Kio/AnuApb | |||
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Genetics
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| Strain Types |
Mutant strain
Coisogenic |
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| Genetic Details |
Transgenic
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| Mode of Inheritance | Dominant | |||
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| Mutant Construction Technique | None | |||
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Phenotype
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| Phenotype Homozygous State | This clone is CD8+ and recognises amino acids 366-374 of the nucleoprotein (NP 366-374) of influenza virus (A/NT/60/68), in the context of Class I MHC Db (Townsend et al., 1986). The usage of V beta 11 makes this TcR reactive to Class II IE molecules and an endogenous ligand recently identified as a product of the endogenous mammary tumour viruses (Mtv) 8, 9, and 11 (Dyson et al., 1991). The development of F5 transgenic T cells and their function in mice of the appropriate MHC (C57BL/10 H-2b, IE-) or in mice expressing Class II MHC IE (e.g., CBA/Ca H-2k and BALB/c H-2d) and the endogenous Mtv ligands. Positive selection of CD8+ T cells expressing the V beta 11 is seen in C57BL/10 transgenic mice (H-2b). Peripheral T cells from these mice are capable of killing target cells in an antigen-dependent manner after a period of in vitro culture with IL-2. In the presence of Class II MHC IE molecules and the endogenous Mtv ligand, most of the single-positive cells carrying the transgenic T-cell receptor are absent in the thymus. Unexpectedly, CD8+ peripheral T-cells in these (H-2k or H-2d) F5 mice are predominantly V beta 11 positive and also have the capacity to kill targets in an antigen-dependent manner. This is true even following backcrossing of the F5 TcR transgene to H-2d scid/scid mice, in which functional rearrangement of endogenous TcR alpha- and beta-chain genes is impaired. | |||
| Original Genetic Background | C57BL/10 | |||
| Genetic Background Currently Maintained | C57BL/10 | |||
| Coat Colour | Black | |||
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Strain identification
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| How is this strain characterised? |
Genotyping
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Fertility and Strain maintenance
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Relevant bibliographic / database references
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General information
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| Associated IP rights? | No | |||
| Does it Model a Human Condition? | No | |||
| Applicable Research Areas |
Immunology and inflammation
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| Keywords |
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| Is the strain available in any form to other researchers | Yes | |||
| APB stock stored as: |
Cryopreserved sperm (from 2 mice)
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| APB stock genotyped verified by: | Unverified | |||
| Are live mice available? | Unknown | |||
| MTA needs to be signed? | Yes | |||
