Mouse Strain information
APB ID 2360
Added 13/12/2007
Last Edited 17/04/2014
Strain name: C57BL/10-Tg(Cd2-TcraF5,CD2-TcrbF5)1Kio/AnuApb
Nickname: TCRF5
Strain designation name: C57BL/10-Tg(Cd2-TcraF5,CD2-TcrbF5)1Kio/AnuApb
Genetics
Strain Types Mutant strain
Coisogenic
Genetic Details Transgenic
Mode of Inheritance Dominant
1.   Transgene: transgene insertion 1, Dimitris Kioussis  
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State This clone is CD8+ and recognises amino acids 366-374 of the nucleoprotein (NP 366-374) of influenza virus (A/NT/60/68), in the context of Class I MHC Db (Townsend et al., 1986). The usage of V beta 11 makes this TcR reactive to Class II IE molecules and an endogenous ligand recently identified as a product of the endogenous mammary tumour viruses (Mtv) 8, 9, and 11 (Dyson et al., 1991). The development of F5 transgenic T cells and their function in mice of the appropriate MHC (C57BL/10 H-2b, IE-) or in mice expressing Class II MHC IE (e.g., CBA/Ca H-2k and BALB/c H-2d) and the endogenous Mtv ligands. Positive selection of CD8+ T cells expressing the V beta 11 is seen in C57BL/10 transgenic mice (H-2b). Peripheral T cells from these mice are capable of killing target cells in an antigen-dependent manner after a period of in vitro culture with IL-2. In the presence of Class II MHC IE molecules and the endogenous Mtv ligand, most of the single-positive cells carrying the transgenic T-cell receptor are absent in the thymus. Unexpectedly, CD8+ peripheral T-cells in these (H-2k or H-2d) F5 mice are predominantly V beta 11 positive and also have the capacity to kill targets in an antigen-dependent manner. This is true even following backcrossing of the F5 TcR transgene to H-2d scid/scid mice, in which functional rearrangement of endogenous TcR alpha- and beta-chain genes is impaired.
Original Genetic Background C57BL/10
Genetic Background Currently Maintained C57BL/10
Coat Colour Black
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? No
Applicable Research Areas Immunology and inflammation
Keywords
  • T cell receptor
  • T cell
  • influenza
  • cytotoxic
  • CD8
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 2 mice)

APB stock genotyped verified by: Unverified
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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