Mouse Strain information
APB ID 201
Added 18/04/2006
Last Edited 24/04/2015
Strain name: B6;129S1-Tgfbr3tm1Stv/Apb
Nickname: TGFbRIII KO
Strain designation name: B6;129S1-Tgfbr3tm1Stv/Apb
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: transforming growth factor, beta receptor III  
Chromosome 5
Affected Gene Symbol Tgfbr3
Protein expression of altered gene: Nil
Genetic alteration of gene: A PGK-Neo expression cassette was inserted in the "sense" orientation into exon 2. Homologous recombination was verified by Southern blot analysis. Two weak bands representing aberrent transcripts were seen in northern blots of mRNA from embryos homozygous from the targeted gene. RT-PCR analysis confirmed that none of these transcripts represented wild-type mRNA and affinity labelling of embryo fibroblasts with TGFbetaI indicated that there was no binding to TGFbetaRIII.
Location on chromosome (bp): 107535589-107718648 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:104637
Synonyms betaglycan, TBRIII
Allele Name targeted mutation 1, Kaye Stenvers
Allele Symbol Tgfbr3tm1Stv
MGI Allele Accession ID MGI:2664514
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Mice homozygous for disruptions in this gene usually die as embryos. The very few individuals that survive are poorly fertile with abnormalities of the spleen, liver, heart, and skeletal system. Embryonic lethal E16.5-E18.5 of heart and liver defects
Original Genetic Background 129S1/Sv x C57BL/6
Genetic Background Currently Maintained C57BL/6 x 129
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Neurobiology
Cell biology
Cardiovascular
Developmental biology
Haematology
Internal/organ
Immunology and inflammation
Keywords
  • heart
  • liver
  • embryonic lethal
  • growth factor
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 21 mice)

APB stock genotyped verified by: The depositor
Are live mice available? Unknown
MTA needs to be signed? Yes


   

 


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